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Comprehensive Leaf Cell Wall Analysis Using Carbohydrate Microarrays Reveals Polysaccharide-Level Variation between Vitis Species with Differing Resistance to Downy Mildew

文献类型： 外文期刊

作者： Gao, Yu 1 ; Yin, Xiangjing 2 ; Jiang, Haoyu 1 ; Hansen, Jeanett 4 ; Jorgensen, Bodil 4 ; Moore, John P. 5 ; Fu, Peinin 1 ;

作者机构： 1.Shanghai Jiao Tong Univ, Ctr Viticulture & Enol, Dept Plant Sci, Sch Agr & Biol, Shanghai 200240, Peoples R China

2.Shanghai Acad Agr Sci, Forestry & Pomol Res Inst, Shanghai 201403, Peoples R China

3.Shanghai Acad Agr Sci, Shanghai Key Lab Protected Hort Technol, Shanghai 201403, Peoples R China

4.Univ Copenhagen, Fac Sci, Dept Plant & Environm Sci, DK-1871 Frederiksberg C, Denmark

5.Stellenbosch Univ, South African Grape & Wine Res Inst, Dept Viticulture & Oenol, ZA-7602 Stellenbosch, South Africa

关键词： cell wall; grapevine; oomycete; polysaccharide microarray; monoclonal antibodies

期刊名称：POLYMERS （ 影响因子：3.426； 五年影响因子：3.636 ）

ISSN：

年卷期： 2021 年 13 卷 9 期

页码：

收录情况： SCI

摘要： The cell wall acts as one of the first barriers of the plant against various biotic stressors. Previous studies have shown that alterations in wall polysaccharides may influence crop disease resistance. In the grapevine family, several native species (e.g., Chinese wild grapevine) show a naturally higher resistance to microbial pathogens than cultivated species (e.g., Vitis vinifera), and this trait could be inherited through breeding. Despite the importance of the cell wall in plant immunity, there are currently no comprehensive cell wall profiles of grapevine leaves displaying differing resistance phenotypes, due to the complex nature of the cell wall and the limitations of analytical techniques available. In this study, the cutting-edge comprehensive carbohydrate microarray technology was applied to profile uninfected leaves of the susceptible cultivar (Vitis vinifera cv. "Cabernet Sauvignon"), a resistant cultivar (Vitis amurensis cv. "Shuanghong") and a hybrid offspring cross displaying moderate resistance. The microarray approach uses monoclonal antibodies, which recognize polysaccharides epitopes, and found that epitope abundances of highly esterified homogalacturonan (HG), xyloglucan (with XXXG motif), (galacto)(gluco)mannan and arabinogalactan protein (AGP) appeared to be positively correlated with the high resistance of Vitis amurensis cv. "Shuanghong" to mildew. The quantification work by gas chromatography did not reveal any significant differences for the monosaccharide constituents, suggesting that polysaccharide structural alterations may contribute more crucially to the resistance observed; this is again supported by the contact infrared spectroscopy of cell wall residues, revealing chemical functional group changes (e.g., esterification of pectin). The identification of certain wall polysaccharides that showed alterations could be further correlated with resistance to mildew. Data from the use of the hybrid material in this study have preliminarily suggested that these traits could be inherited and may be applied as potential structural biomarkers in future breeding work.

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