Anti-idiotypic single-chain variable fragment antibody partially mimic the functionally spatial structure of Cry2Aa toxin
文献类型： 外文期刊
作者： Lin, Manman 1 ; Liu, Yuan 1 ; Zhang, Xiao 1 ; Zhong, Jianfeng 1 ; Hu, Xiaodan 1 ; Xu, Chongxin 1 ; Xie, Yajing 1 ; Zhang, 1 ;
作者机构： 1.Jiangsu Acad Agr Sci, Inst Food Safety & Nutr,Minist Sci & Technol,Mini, Jiangsu Key Lab Food Qual & Safety,State Key Lab, Key Lab Control Technol & Stand Agroprod Safety &, Nanjing 210014, Peoples R China
2.Univ KwaZulu Natal, Coll Agr Engn & Sci, Sch Life Sci, Discipline Microbiol, Westville Campus,Private Bag X54001, ZA-4000 Durban, South Africa
3.Jiangsu Univ, Sch Food & Biol Engn, Zhenjiang 212013, Jiangsu, Peoples R China
关键词： Cry2Aa toxin; Anti-idiotypic antibody; Single-chain variable fragment (scFv)
期刊名称：ANALYTICAL BIOCHEMISTRY （ 影响因子：2.877； 五年影响因子：2.549 ）
ISSN： 0003-2697
年卷期： 2021 年 625 卷
页码：
收录情况： SCI
摘要： The anti-idiotypic antibody is widely used in the field of immunology to simulate structural features or even induce the biological activity of antigens. In this study, we obtained seven anti-idiotypic single-chain variable fragments (scFv) antibodies of Cry2Aa toxin from a phage-displayed mutant library constructed using errorprone PCR technique. A mutant designated 2-12B showed the best binding ability amongst all anti-idiotypic scFv isolates to Plutella xylostella brush border membrane vesicles (BBMVs). 2-12B and Cry2Aa toxin shared a potential receptor of polycalin in P. xylostella BBMVs. Homology modeling and molecular docking demonstrated that 2-12B and Cry2Aa toxin have seven common binding amino acid residues in polycalin. Insect bioassay results suggested that 2-12 had insecticidal efficacy against P. xylostella larvae. These results indicated that the Cry2Aa anti-idiotypic scFv antibody 2-12B partially mimicked the structure and function of Cry2Aa toxin. The anti-idiotypic scFv antibody provides the basic material for the future study of surrogate molecules or new insecticidal materials.
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