Overexpression of MpbHLH transcription factor, an encoding ICE1-like protein, enhances Foc TR4-resistance of Cavendish banana
文献类型： 外文期刊
作者： Li, Haochen 1 ; Hu, Chunhua 1 ; Xie, Aifeng 1 ; Wu, Shaoping 1 ; Bi, Fangcheng 1 ; Dong, Tao 1 ; Li, Chunyu 1 ; Deng, Guiming 1 ; He, Weidi 1 ; Gao, Huijun 1 ; Sheng, Ou 1 ; Yi, Ganjun 1 ; Yang, Qiao-Song 1 ; Dou, Tongxin 1 ;
作者机构： 1.Guangdong Acad Agr Sci, Minist Agr & Rural Affairs,Inst Fruit Tree Res, Guangdong Key Lab Trop & Subtrop Fruit Tree Res, Key Lab South Subtrop Fruit Biol & Genet Resource, Guangzhou, Peoples R China
2.South China Agr Univ, Coll Plant Protect, Guangzhou, Peoples R China
3.South China Agr Univ, Coll Life Sci, Guangzhou, Peoples R China
4.Hunan Agr Univ, Coll Hort, Changsha, Peoples R China
关键词： Banana; Fusarium wilt; MpbHLH; POD; Transcription factor; ICE1
期刊名称：SCIENTIA HORTICULTURAE （ 影响因子：4.342； 五年影响因子：4.342 ）
ISSN： 0304-4238
年卷期： 2022 年 291 卷
页码：
收录情况： SCI
摘要： Banana (Musa spp.) is an important tropical fruit with high economic value, however, Fusarium wilt disease is a critical factor affecting the sound development of the banana industry. Different cultivars of banana exhibit a high degree of genetic variability for resistance to Fusarium wilt. Compared with Cavendish banana (Musa spp. AAA group), Dajiao (Musa spp. ABB group) has superior disease resistance and provides an excellent test material for the isolation and characterization of disease resistance genes. In this study, a Dajiao candidate gene, MpbHLH (basic helix-loop-helix), an encoding ICE1-like protein transcription factor (TF), was cloned and characterized. Real-time quantitative PCR (qPCR) results revealed that MpbHLH transcript levels were strongly induced under cold treatment, salt, and dehydration. Overexpression of MpbHLH in Foc TR4-sensitive Cavendish banana showed that the transgenic lines had superior resistance to Fusarium wilt compared with the wild-type (WT). Proteomic analysis showed that phenylalanine ammonia lyase (PAL) and peroxidase (POD) were highly expressed in transgenic plants. Physiological experiments further revealed that transgenic plants, especially their roots, had high POD activity. Taken together, overexpression of MpbHLH in banana may extensively activate stress-related genes. In particular, the activity of POD increases the resistance of transgenic banana to Fusarium wilt by enhancing the reactive oxygen species (ROS) scavenging system of a transgenic plant.
- 相关文献
作者其他论文 更多>>
-
High-quality genome assemblies for two Australimusa bananas (Musa spp.) and insights into regulatory mechanisms of superior fiber properties
作者：Zhou, Run;Zhan, Ni;He, Weidi;Deng, Guiming;Dou, Tongxin;Hu, Chunhua;Bi, Fangcheng;Gao, Huijun;Dong, Tao;Liu, Siwen;Li, Chunyu;Yang, Qiaosong;Yi, Ganjun;Sheng, Ou;Zhou, Run;Wang, Shuo;Zhu, Xi-Tong;Xie, Wen-Zhao;Zheng, Yu-Yu;Wang, Lingqiang;Song, Jia-Ming;Chen, Ling-Ling;Dang, Jiangbo;Guo, Qigao
关键词：Australimusabananasx; T genome; Musaceae evolution; structural variations; fiber
-
Identification and characterization of two P450 enzymes from Citrus sinensis involved in TMTT and DMNT biosyntheses and Asian citrus psyllid defense
作者：Sun, Xueli;Hu, Chunhua;Yi, Ganjun;Zhang, Xinxin;Sun, Xueli;Hu, Chunhua;Yi, Ganjun;Zhang, Xinxin;Sun, Xueli
关键词：
-
MaSMG7-Mediated Degradation of MaERF12 Facilitates Fusarium oxysporum f. sp. cubense Tropical Race 4 Infection in Musa acuminata
作者：Huang, Huoqing;Liu, Siwen;Huo, Yile;Liu, Yushan;Yi, Ganjun;Li, Chunyu;Liu, Siwen;Yi, Ganjun;Li, Chunyu;Liu, Siwen;Yi, Ganjun;Li, Chunyu;Tian, Yuzhen
关键词：banana wilt; Fusarium oxysporum f. sp. cubense tropical race 4; MaERF12; MaSMG7; cell death
-
Two haplotype-resolved genome assemblies for AAB allotriploid bananas provide insights into banana subgenome asymmetric evolution and Fusarium wilt control
作者：Xie, Wen-Zhao;He, Weidi;Bi, Fangcheng;Li, Yaoyao;Dou, Tongxin;Deng, Guiming;Liu, Siwen;Li, Chunyu;Yang, Qiaosong;Hu, Chunhua;Gao, Huijun;Dong, Tao;Yi, Ganjun;Sheng, Ou;Xie, Wen-Zhao;Zhou, Run;Guo, Yi-Xiong;Zhang, Wenhui;Sanz-Jimenez, Pablo;Zhou, Zhi-Wei;Feng, Jia-Wu;Zhang, Jianwei;Yuan, Min-Hui;Zhu, Xi-Tong;Xu, Xin-Dong;Zhou, Zu-Wen;Cai, Wenguo;Song, Jia-Ming;Chen, Ling-Ling;Dang, Jiangbo;Guo, Qigao
关键词：bananas; Plantain; Silk; asymmetric evolution; homologous exchange; Fusarium wilt
-
Near-freezing temperature suppresses avocado (Persea americana Mill.) fruit softening and chilling injury by maintaining cell wall and reactive oxygen species metabolism during storage
作者：Qin, Jian;Shao, Xuehua;Lai, Duo;Xiao, Weiqiang;Zhuang, Qingli;Dong, Tao;Chen, Xi;Tang, Xiuhua;Wang, Wenlin
关键词：Avocado; Near -freezing temperature; Firmness; Chilling injury; Cell wall; Reactive oxygen species
-
A double methylene blue labeled single-stranded DNA and hairpin DNA coupling biosensor for the detection of Fusarium oxysporum f. sp. cubense race 4
作者：Zhang, Zhihong;Ou, Xiangying;Ma, Lizhe;Yang, Zhou;Duan, Jieli;Yang, Zhou;Duan, Jieli;Yang, Zhou;Li, Chunyu
关键词：Banana fusarium wilt; Electrochemical biosensor; AuNPs/rGO nanocomposite; ssDNA-hDNA coupling system; Screen-printed electrodes
-
A strong "on-off" electrochemical biosensor for detecting dicer-like molecules in Fusarium oxysporum f. sp. cubense race 4 (Foc4)
作者：Zhang, Zhihong;Ma, Lizhe;Jiang, Tingting;Yang, Zhou;Duan, Jieli;Yang, Zhou;Duan, Jieli;Yang, Zhou;Li, Chunyu
关键词：Banana Fusarium wilt; Dicer -like; Electrochemical adapted sensor; Strong switching signal; ssDNA-hDNA coupled system
