Overexpression of MpbHLH transcription factor, an encoding ICE1-like protein, enhances Foc TR4-resistance of Cavendish banana
文献类型： 外文期刊
作者： Li, Haochen 1 ; Hu, Chunhua 1 ; Xie, Aifeng 1 ; Wu, Shaoping 1 ; Bi, Fangcheng 1 ; Dong, Tao 1 ; Li, Chunyu 1 ; Deng, Guiming 1 ; He, Weidi 1 ; Gao, Huijun 1 ; Sheng, Ou 1 ; Yi, Ganjun 1 ; Yang, Qiao-Song 1 ; Dou, Tongxin 1 ;
作者机构： 1.Guangdong Acad Agr Sci, Minist Agr & Rural Affairs,Inst Fruit Tree Res, Guangdong Key Lab Trop & Subtrop Fruit Tree Res, Key Lab South Subtrop Fruit Biol & Genet Resource, Guangzhou, Peoples R China
2.South China Agr Univ, Coll Plant Protect, Guangzhou, Peoples R China
3.South China Agr Univ, Coll Life Sci, Guangzhou, Peoples R China
4.Hunan Agr Univ, Coll Hort, Changsha, Peoples R China
关键词： Banana; Fusarium wilt; MpbHLH; POD; Transcription factor; ICE1
期刊名称：SCIENTIA HORTICULTURAE （ 影响因子：4.342； 五年影响因子：4.342 ）
ISSN： 0304-4238
年卷期： 2022 年 291 卷
页码：
收录情况： SCI
摘要： Banana (Musa spp.) is an important tropical fruit with high economic value, however, Fusarium wilt disease is a critical factor affecting the sound development of the banana industry. Different cultivars of banana exhibit a high degree of genetic variability for resistance to Fusarium wilt. Compared with Cavendish banana (Musa spp. AAA group), Dajiao (Musa spp. ABB group) has superior disease resistance and provides an excellent test material for the isolation and characterization of disease resistance genes. In this study, a Dajiao candidate gene, MpbHLH (basic helix-loop-helix), an encoding ICE1-like protein transcription factor (TF), was cloned and characterized. Real-time quantitative PCR (qPCR) results revealed that MpbHLH transcript levels were strongly induced under cold treatment, salt, and dehydration. Overexpression of MpbHLH in Foc TR4-sensitive Cavendish banana showed that the transgenic lines had superior resistance to Fusarium wilt compared with the wild-type (WT). Proteomic analysis showed that phenylalanine ammonia lyase (PAL) and peroxidase (POD) were highly expressed in transgenic plants. Physiological experiments further revealed that transgenic plants, especially their roots, had high POD activity. Taken together, overexpression of MpbHLH in banana may extensively activate stress-related genes. In particular, the activity of POD increases the resistance of transgenic banana to Fusarium wilt by enhancing the reactive oxygen species (ROS) scavenging system of a transgenic plant.
- 相关文献
作者其他论文 更多>>
-
Precise A
T-to-G C Base Editing in Banana 作者：Wu, Shaoping;Wu, Shaoping;Hu, Chunhua;Dou, Tongxin;Qin, Fei;Yin, Binhui;Bi, Fangcheng;Yang, Qiaosong;Yi, Ganjun;Hu, Chunhua;Dou, Tongxin;Bi, Fangcheng;Yang, Qiaosong;Yi, Ganjun;Zhu, Haocheng;Liu, Jinxing;Zhu, Haocheng;Yin, Binhui
关键词：Banana; Adenine base editor;
MaPDS ; Paraffin section; Deep amplicon sequencing -
Fusaric acid-mediated S-glutathionylation of MaAKT1 channel confers the virulence of Foc TR4 to banana
作者：Zhang, Jun;Zhang, Zhi-Ren;Zhang, Jun;Yao, Xiaoqiang;Liu, Siwen;Yang, Wenlong;Xie, Yanling;Shao, Chuange;Li, Chunyu;Liu, Siwen;Li, Chunyu;Liu, Siwen;Li, Chunyu;Yao, Xiaoqiang
关键词：
-
Identification of Aspartate Aminotransferase FocAST2 as a Novel Target of Albendazole in Fusarium oxysporum f. sp. cubense TR4
作者：Liu, Yushan;Liu, Siwen;Peng, Cheng;Huang, Huoqing;Zhang, Wei;Huo, Yile;Yi, Ganjun;Li, Chunyu;Zeng, Weiqing
关键词：banana;
Foc TR4; albendazole; target; aspartate transaminase -
Transcriptome sequence reveal the roles of MaGME777 and MabHLH770 in tolerance in Musa acuminata
作者：Wei, Yuchen;Zhang, Feng;Dong, Huizhen;Huang, Xiaosan;Li, Yaoyao;Bi, Fangcheng;Dou, Tongxin;Li, Yaoyao;Bi, Fangcheng;Dou, Tongxin;Li, Yaoyao;Bi, Fangcheng;Dou, Tongxin;Zhang, Feng;Dong, Huizhen;Huang, Xiaosan;Shi, Jingfang;Xie, Xueyi
关键词：Cavendish banana; Drought stress; RNA-Seq; WGCNA; BHLH
-
Integrated genomic and transcriptomic analysis reveals the mechanisms underlying leaf variegation in 'Gonggan' mandarin
作者：Shi, Cong;Gu, Miaofeng;You, Congjun;Bao, Sihan;Xie, Shuangling;Gong, Jinli;Sun, Xuepeng;Shi, Cong;Huang, Yongjing;Deng, Guiming;Wu, Pingzhi;Wu, Wen;Zhu, Congyi;Zeng, Jiwu
关键词：Plant genome; leaf variegation; Chloroplast development; Citrus; Genome assembly; Transcriptome; Gene co-expression network
-
Genome-Wide Profiling of bZIP Transcription Factors and FocbZIP11's Impact on Fusarium TR4 Pathogenicity
作者：Xie, Yanling;Huang, Huoqing;Huo, Yile;Yang, Wenlong;Li, Yuqing;Liu, Siwen;Li, Chunyu;Xie, Yanling;Xie, Yanling;Liu, Siwen;Li, Chunyu;Liu, Siwen;Li, Chunyu
关键词：
Fusarium oxysporum f. sp.cubense tropical race 4; fungal stress adaptation; plant-pathogen interactions; virulence -
Postharvest Application of Myo-Inositol Extends the Shelf-Life of Banana Fruit by Delaying Ethylene Biosynthesis and Improving Antioxidant Activity
作者：Hu, Lingyu;Li, Yi;Zhou, Kun;Shi, Kaili;Niu, Yi;Qu, Feng;Zhang, Shenglin;He, Weidi;Wu, Yuanli;He, Weidi;Wu, Yuanli
关键词：banana ripening; shelf-life;
myo -inositol; ethylene biosynthesis; reactive oxygen species; cell wall loosening
