Overexpression and characterization of a novel GH4 galactosidase with beta-galactosidase activity from Bacillus velezensis SW5
文献类型： 外文期刊
作者： Li, Na 1 ; Liu, Yang 1 ; Wang, Changyu 1 ; Weng, Peifang 1 ; Wu, Zufang 1 ; Zhu, Yazhu 3 ;
作者机构： 1.Ningbo Univ, Coll Food & Pharmaceut Sci, Ningbo 315211, Peoples R China
2.Fujian Acad Agr Sci, Inst Qual Stand & Testing Technol Agroprod, Fuzhou 350003, Peoples R China
3.Zhejiang Int Maritime Coll, Zhoushan 316021, Peoples R China
关键词： Bacillus velezensis; galactosidase; enzymatic properties; lactase hydrolysis
期刊名称：JOURNAL OF DAIRY SCIENCE （ 影响因子：4.034； 五年影响因子：4.354 ）
ISSN： 0022-0302
年卷期： 2021 年 104 卷 9 期
页码：
收录情况： SCI
摘要： A novel galactosidase gene (gal3149) was identified from Bacillus velezensis SW5 and heterologously expressed in Escherichia coli. BL21 (DE3). The novel galactosidase, Gal3149, encoded by gal3149 in an open reading frame of 1,299 bp, was 433 amino acids in length. Protein sequence analysis showed that Gal3149 belonged to family 4 of glycoside hydrolases (GH4). Gal3149 displayed higher enzyme activity for the substrate 2-nitrophenyl-beta-D-gal actopyranoside (oNPG) than for 4-nitrophenyl-alpha-D-galactopyranoside (pNP alpha G). This is the first time that an enzyme belonging to GH4 has been shown to exhibit beta-galactosidase activity. Gal3149 showed optimal activity at pH 8.0 and 50 degrees C, and exhibited excellent thermal stability, with retention of 50% relative activity after incubation at a temperature range of 0 to 50 degrees C for 48 h. Gal3149 activity was significantly improved by K+ and Na+, and was strongly or completely inhibited by Ag+, Zn2+, Tween-80, Cu2+, carboxymethyl cellulose, and oleic acid. The rate of hydrolyzed lactose in 1 mL of milk by 1 U of Gal3149 reached about 50% after incubation for 4 h. These properties lay a solid foundation for Gal3149 in application of the lactose-reduced dairy industry.
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