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Characteristics and functions of interleukin 21 in channel catfish (Ictalurus punctatus)

文献类型： 外文期刊

作者： He, Hao 1 ; Zhu, Xia 2 ; Deng, Ping 3 ; Huang, Yucong 4 ; Wang, Hui 5 ; Dong, Jing 2 ; Yang, Qiuhong 2 ; Ai, Xiaohui 2 ; Yang, Yibin 2 ;

作者机构： 1.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China

2.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan 430223, Peoples R China

3.Hubei Acad Agr Sci, Wuhan 430064, Peoples R China

4.Guangdong Ocean Univ, Coll Fisheries, Guangdong Prov Key Lab Aquat Anim Dis Control & Hl, Zhanjiang 524088, Peoples R China

5.Yancheng Acad Fishery Sci, Yancheng Biol Engn Higher Vocat Technol Sch, Yancheng 224008, Peoples R China

关键词： Channel catfish; IL-21; Immune response; Inflammatory factors; Gene expression

期刊名称：MICROBIAL PATHOGENESIS （ 影响因子：3.5； 五年影响因子：3.6 ）

ISSN： 0882-4010

年卷期： 2025 年 202 卷

页码：

收录情况： SCI

摘要： Interleukin-21 (IL-21) is mainly produced by CD4+ T cells and NKT cells, belonging to the gamma chain (gamma c) family. It promotes the proliferation, activation, and differentiation of immune cells and activates the JAK-STAT, MAPK signaling, and PI3K pathways to regulate immune responses, crucially impacting anti-infectious inflammatory responses. In this study, IL-21 was successfully identified in channel catfish (Ictalurus punctatus, Ip), with a coding sequence (CDS) length of 438 bp encoding a 145-amino acid (aa) protein. The signal peptide comprised 19 aa, while the mature peptide consists of 126 aa, featuring four alpha-helix structures and two pairs of disulfide bonds from four conserved cysteine residues. qPCR data revealed highest IpIL-21 expression in channel catfish gills and spleen. Bacterial and viral infections upregulated IpIL-21 expression across multiple tissues. Poly(I:C) specifically enhanced IpIL-21 expression in channel catfish kidney cells (CCK) at 48 h post-infection (hpi). In vitro, recombinant IpIL-21 (rIpIL-21) protein induced upregulation of IL-10, IL-21, IL-1 beta, and STAT3 expression in CCK, while inhibiting IL-22 and IL-26 expression. IL-6 and IL-20 expressions were inhibited at low doses and induced at high doses, demonstrating a dose-dependent pattern. These findings underscored IpIL-21's significance as a critical immune factor in channel catfish, pivotal for the antibacterial inflammatory response defense system.

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