Biochemical characterization of a GH10 xylanase from the anaerobic rumen fungus Anaeromyces robustus and application in bread making
文献类型： 外文期刊
作者： Wen, Sitao 1 ; Wu, Guogan 2 ; Wu, Huawei 1 ;
作者机构： 1.Yangtze Univ, Dept Life & Sci, 1 South Loop Rd, Jingzhou 434025, Peoples R China
2.Shanghai Acad Agr Sci, Biotechnol Res Inst, 2901 Bei Zhai Rd, Shanghai 201106, Peoples R China
关键词： Xylanase; Pichia pastoris; Ruminal microorganisms; Anaeromyces robustus; Bread
期刊名称：3 BIOTECH （ 影响因子：2.406； 五年影响因子：3.203 ）
ISSN： 2190-572X
年卷期： 2021 年 11 卷 9 期
页码：
收录情况： SCI
摘要： Anaeromyces robustus is an anaerobic rumen microorganism which can produce plant cell wall degrading enzymes. In this study, a new GH10 xylanase gene xylAr10 from A. robustus was identified, cloned and expressed in Pichia pastoris GS115. The recombinant protein ArXyn10 was characterized after being purified by Ni-NTA. The optimal pH and temperature of ArXyn10 was determined at 5.5 and 40 degrees C, respectively. ArXyn10 was stable at the pH range of 4.0-8.0, and could maintain high stability from 35 to 45 degrees C. The hydrolysis products released from beechwood xylan by ArXyn10 showed chromatographic mobility similar to xylobiose and xylotriose according to thin-layer chromatography analysis. It was shown that the addition of 7.5 mg of ArXyn10 in 100 g high-gluten wheat flour during bread making could increase the reducing sugar content by 10.80%, indicating that xylo-oligosaccharides were produced. With the addition of ArXyn10, the hardness and chewiness of the bread decreased and the quality was improved. The new discovered xylanase ArXyn10 have potential application prospect in bread making.
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