syn-tasiRnas targeting the coat protein of potato virus Y confer antiviral resistance in Nicotiana benthamiana
文献类型： 外文期刊
作者： Zhao, Xingyue 1 ; Gao, Qian 1 ; Wang, Haijuan 1 ; Yue, Jianying 1 ; An, Derong 2 ; Li, Bin 3 ; Yan, Fangfang 4 ; Carmen, Simon-Mateo 5 ; Zhao, Yuanzheng 6 ; Zhou, Hongyou 1 ; Zhao, Mingmin 1 ;
作者机构： 1.Inner Mongolia Agr Univ, Coll Hort & Plant Protect, 306 Zhaowuda Rd, Hohhot, Peoples R China
2.Northwest A&F Univ, Coll Plant Protect, Yangling, Peoples R China
3.Sichuan Prov Co Tobacco Corp China, Dept Tabacco Prod, Chengdu, Peoples R China
4.Panzhihua City Co Sichuan Prov Co Tobacco Corp Chi, Panzhihua, Sichuan, Peoples R China
5.CSIC, Ctr Nacl Biotecnol, Madrid, Spain
6.Inner Mongolia Acad Agr & Anim Husb Sci, Dept Plant Protect, Hohhot, Peoples R China
7.Key Lab Dev & Resource Utilizat Biol Pesticide Inn, Dept Plant protect, Hohhot, Peoples R China
关键词： Potato; potato virus Y; trans-acting small interfering RNA; synthetic sirnas
期刊名称：PLANT SIGNALING & BEHAVIOR （ 影响因子：2.9； 五年影响因子：2.9 ）
ISSN： 1559-2316
年卷期： 2024 年 19 卷 1 期
页码：
收录情况： SCI
摘要： Trans-acting small interfering RNAs (tasiRNAs) are 21-nt phased (phased siRNAs) resulting from successive DCL-catalyzed processing from the end of a double-stranded RNA substrate originating from the RDR of an AGO-catalyzed cleaved RNA at a micro RNA target site. Plant tasiRNAs have been synthesized to produce synthetic tasiRNAs (syn-tasiRNAs) targeting viral RNAs that confer viral resistance. In this study, we engineered syn-tasiRNAs to target potato virus Y (PVY) infection by replacing five native siRNAs of TAS1c with 210-bp fragments from the coat protein (CP) region of the PVY genome. The results showed that the transient expression of syn-tasiR-CPpvy2 in Nicotiana benthamiana (N. benthamiana) plants conferred antiviral resistance, supported by the absence of PVY infection symptoms and viral accumulation. This indicated that syn-tasiR-CPpvy2 successfully targeted and silenced the PVY CP gene, effectively inhibiting viral infection. syn-tasiR-CPpvy1 displayed attenuated symptoms and decreased viral accumulation in these plants However, severe symptoms of PVY infection and a similar amount of viral accumulation as the control were observed in plants expressing syn-tasiR-CPpvy3. syn-tasiR-CPpvy/pvx, which targets both PVY and potato virus X (PVX), was engineered using a single precursor. After the transient expression of syn-tasiR-CPpvy/pvx3 and syn-tasiR-CPpvy/pvx5 in N. benthamiana, the plants were resistant to both PVY and PVX. These results suggested that engineered syn-tasiRNAs could not only specifically induce antiviral resistance against one target virus but could also be designed for multi-targeted silencing of different viruses, thereby preventing complex virus infection in plants.
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