The Bile Acid Metabolism of Intestinal Microorganisms Mediates the Effect of Different Protein Sources on Muscle Protein Deposition in Procambarus clarkii
文献类型： 外文期刊
作者： Xu, Xiaodi 1 ; Zheng, Xiaochuan 1 ; Zhou, Qunlan 1 ; Sun, Cunxin 1 ; Wang, Aimin 3 ; Zhu, Aimin 4 ; Zhang, Yuanyuan 5 ; Liu, Bo 1 ;
作者机构： 1.Nanjing Agr Univ, Wuxi Fisheries Coll, Wuxi 214128, Peoples R China
2.Chinese Acad Fishery Sci CAFS, Freshwater Fisheries Res Ctr FFRC, Key Lab Genet Breeding Aquat Anim & Aquaculture Bi, Wuxi 214081, Peoples R China
3.Yancheng Inst Technol, Coll Marine & Biol Engn, Yancheng 224051, Peoples R China
4.Yancheng Acad Fishery Sci, Yancheng 224051, Peoples R China
5.Shandong Freshwater Fisheries Res Inst, Jinan 250013, Peoples R China
关键词： Procambarus clarkii; protein source; protein deposition; taurochenodeoxycholic acid; intestinal microbiota
期刊名称：MICROORGANISMS （ 影响因子：4.2； 五年影响因子：4.6 ）
ISSN：
年卷期： 2025 年 13 卷 1 期
页码：
收录情况： SCI
摘要： The most economically important trait of the Procambarus clarkii is meat quality. Protein deposition is essential in muscle growth and nutritional quality formation. The effects and potential mechanisms of feed protein sources on crustaceans' muscle protein deposition have not been elucidated. This study established an all-animal protein source (AP) and an all-plant protein source group (PP), with a feeding period of 8 weeks (four replicates per group, 45 individuals per replicate). The results demonstrated that muscle protein deposition, muscle fiber diameter, and hardness were significantly higher in the PP group (p < 0.05). The transcript levels of genes involved in protein synthesis were notably upregulated, while those of protein hydrolysis and negative regulators of myogenesis notably downregulated in PP group (p < 0.05). Furthermore, protein sources shaped differential intestinal microbiota composition and microbial metabolites profiles, as evidenced by a significant decrease in g_Bacteroides (p = 0.030), and a significant increase in taurochenodeoxycholic acid (TCDCA) in PP group (p = 0.027). A significant correlation was further established by Pearson correlation analysis between the g_Bacteroides, TCDCA, and genes involved in the MSTN-mediated protein deposition pathway (p < 0.05). In vitro anaerobic fermentation confirmed the ability of the two groups of intestinal flora to metabolically produce differential TCDCA (p = 0.038). Our results demonstrated that the 'Bacteroides-TCDCA-MSTN' axis may mediate the effects of different protein sources on muscle development and protein deposition in P. clarkii, which was anticipated to represent a novel target for the muscle quality modulation in crustaceans.
- 相关文献
作者其他论文 更多>>
-
Individual and combined effects of ZnO nanoparticles and cinnamon essential oil on Salmonella typhimurium in milk-based beverage
作者：Zhang, Yuanyuan;Lu, Feng;Liang, Ruohan;Pu, Xiaodan;Zhang, Xuzhi;Zhang, Yuanyuan;Lu, Feng;Liang, Ruohan;Pu, Xiaodan;Yang, Qianqian;Wang, Xiaoyang;Zhang, Xuzhi;Zhang, Dahai;Chen, Zhixiang
关键词：Antibacterial substances; ZnO nanoparticles; Cinnamon essential oil; Antibacterial activity; Milk-based beverage
-
Electrical Microbial Growth Analyzer-Based Method for Quantifying Viable Bacteria in Oyster Sauce
作者：Liang, Ruohan;Lu, Feng;Zhang, Yuanyuan;Pu, Xiaodan;Wang, Xiaoyang;Zhu, Xueting;Yang, Qianqian;Zhang, Xuzhi;Liang, Ruohan;Lu, Feng;Zhang, Yuanyuan;Pu, Xiaodan;Zhang, Dahai;Chen, Zhixiang
关键词：Foodborne bacteria; Quantification; Culture-based method; Oyster sauce;
S. aureus ;V. parahaemolyticus -
Multi-Omics Analysis Provides New Insights into the Interplay Between Gut Microbiota, Fatty Acid Metabolism, and Immune Response in Cultured and Wild Coilia nasus from the Yangtze River Area in China
作者：Yang, Chang;Liu, Kai;Deng, Yanmin;Cao, Shiqian;Zhou, Qunlan;Liu, Kai;Zhou, Qunlan;Liu, Kai;Wang, Qianhui;Zhou, Qunlan
关键词：estuarine tapertail anchovy; polyunsaturated fatty acid; immunity; muscle transcriptome; 16S rRNA sequencing
-
Taurochenodeoxycholic Acid Improves Growth, Physiology, Intestinal Microbiota, and Muscle Development in Red Swamp Crayfish (Procambarus clarkii)
作者：Xu, Xiaodi;Zheng, Xiaochuan;Song, Changyou;Liu, Xin;Zhou, Qunlan;Sun, Cunxin;Liu, Bo;Xu, Xiaodi;Zheng, Xiaochuan;Song, Changyou;Liu, Xin;Zhou, Qunlan;Sun, Cunxin;Liu, Bo;Wang, Aimin;Zhu, Aiming
关键词：TCDCA; muscle quality; protein deposition; gut microbiota;
Procambarus clarkii -
Beneficial effects of Haematococcus pluvialis on growth and hepatopancreas health status in crayfish (Procambarus clarkii) via remodeling the gut microbial metabolic functions
作者：Wang, Aimin;Hui, Wenjie;Tian, Hongyan;Zhang, Wuxiao;Xia, Silei;Liu, Fei;Yang, Wenping;Yu, Yebing;Hui, Wenjie;Deng, Xin;Deng, Xin;Liu, Bo;Shao, Peng;Zhu, Aimin;Shao, Peng
关键词：Procambarus clarkii; Haematococcus pluvialis; Growth; Hepatopancreas health; Gut microbiota
-
Effect of Replacement of Fish Meal With Cottonseed and Blood Meals on Growth, Serum Biochemistry, Body Composition, and Antioxidant Capacity in GIFT Tilapia Juveniles (Oreochromis niloticus)
作者：Liu, Xin;Liu, Bo;Zhou, Qunlan;Liu, Bo;Liu, Bo;Zhou, Qunlan;Sun, Cunxin;Zheng, Xiaochuan;Liu, Bo;Liu, Bo;Zhou, Qunlan;Sun, Cunxin;Zheng, Xiaochuan;Liu, Bo
关键词：blood chemistry; fish meal replacement; growth metrics; proximate composition analysis
-
The chromosomal-level genome assembly and annotation of pen shell Atrina pectinata
作者：Li, Zhuanzhuan;Chen, Xi;Ma, Peizhen;Liu, Zhihong;Sun, Xiujun;Zhou, Liqing;Wu, Biao;Li, Zhuanzhuan;Chen, Xi;Ma, Peizhen;Liu, Zhihong;Sun, Xiujun;Zhou, Liqing;Wu, Biao;Liu, Bo;Ren, Jianfeng;Zheng, Yanxin;Yu, Tao
关键词：
