文献类型： 外文期刊

作者： Huang, Xiying ¹ ; Zhang, Runji ¹ ; Yang, Qinyan ¹ ; Li, Xinzhu ² ; Xiang, Quanju ¹ ; Zhao, Ke ¹ ; Ma, Menggen ¹ ; Yu, Xiumei ¹ ; Chen, Qiang ¹ ; Zeng, Xianfu ² ; Zhang, Lujun ³ ; Penttinen, Petri ¹ ; Gu, Yunfu ¹ ;

作者机构： 1.Sichuan Agr Univ, Coll Resources, Dept Microbiol, Chengdu, Peoples R China

2.Chengdu Acad Agr & Forestry Sci, Chengdu, Peoples R China

3.Shanghai Acad Agr Sci, Shanghai, Peoples R China

关键词： Lentinula edodes; composted sawdust; transcriptomics; proteomics

期刊名称：MSYSTEMS （ 影响因子：7.324； 五年影响因子：9.039 ）

ISSN： 2379-5077

年卷期： 2022 年 7 卷 1 期

页码：

收录情况： SCI

摘要： In mushroom cultivation, composting the substrate can make the nutrients more easily absorbed by hyphae due to the degradation of lignin, cellulose, and other organic matter. However, the effects of cultivating Lentinula edodes on composted substrate and the related molecular mechanisms have not been studied systemically. We applied transcriptomics, qRT-PCR, and proteomics to study L. edodes cultivated on substrates with fresh (CK) and composted (ND) sawdust, focusing on the brown film formation stage. The time of brown film formation was shorter and the mycelium growth rate and crude polysaccharide content of the brown film were higher in ND than in CK. The faster growth rate in ND may have been due to the higher nitrogen content in ND than in CK. Among the 9,455 genes annotated using transcriptomics, 96 were upregulated and 139 downregulated in ND compared with CK. Among the 2,509 proteins identified using proteomics sequencing, 74 were upregulated and 113 downregulated. In the KEGG pathway analyses, both differentially expressed genes and proteins were detected in cyanoamino acid metabolism, inositol phosphate metabolism, pentose and glucuronate interconversions, phosphatidylinositol signaling system, RNA polymerase, starch and sucrose metabolism, and tyrosine metabolism pathways. A large number of differentially expressed genes (DEGs) related to aromatic amino acid metabolic and biosynthetic process were upregulated in ND. Most of the DEGs annotated to carbohydrate active enzymes were downregulated in L. edodes growing on composted sawdust containing substrate, possibly due to the lower hemicellulose and cellulose contents in the composted sawdust. The results suggested that using composted substrate may decrease the cultivation time and improve the quality of L. edodes and revealed the underlying molecular mechanisms. IMPORTANCE Composted substrates are not commonly used in the cultivation of Lentinula edodes, thus the effects of cultivating L. edodes on composted substrate and the related molecular mechanisms have not been studied systemically. We studied L. edodes cultivated on substrates with fresh (CK) and composted (ND) sawdust, focusing on the brown film formation stage, and determined the composting related differences in the substrate and in the growth and gene expression of L. edodes. Cultivation on composted substrate was beneficial and showed potential for decreasing the cultivation time and improving the quality of L. edodes. Analyzing the expression levels of genes and proteins in brown film revealed gene and metabolism pathway level changes that accompanied the cultivation on composted substrate.