文献类型： 外文期刊

作者： Liu, Dehui ¹ ; Li, Xue ¹ ; Wang, Lei ³ ; Pei, Quanbang ³ ; Zhao, Jincai ³ ; Sun, De ⁶ ; Ren, Qianben ³ ; Tian, Dehong ¹ ; Han, Buying ¹ ; Jiang, Hanjing ⁵ ; Zhang, Wenkui ³ ; Wang, Song ¹ ; Tian, Fei ¹ ; Liu, Sijia ¹ ; Zhao, Kai ¹ ;

作者机构： 1.Chinese Acad Sci, Northwest Inst Plateau Biol, Qinghai Prov Key Lab Anim Ecol Genom, Key Lab Adaptat & Evolut Plateau Biota, 23 Xinning Rd, Xining 810001, Qinghai, Peoples R China

2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

3.Qinghai Sheep Breeding & Promot Serv Ctr, Gangcha 812300, Qinghai, Peoples R China

4.Heilongjiang Acad Agr Sci, Branch Anim Husb & Vet, Qiqihar 161005, Peoples R China

5.Qinghai Livestock & Poultry Genet Resources Protec, Xining 810000, Peoples R China

6.Anim Husb & Vet Stn Huzhu Cty Qinghai Prov, Huzhu 810500, Qinghai, Peoples R China

关键词： Tibetan sheep; Re-sequencing; GWAS; Body size traits; SNP

期刊名称：BMC GENOMICS （ 影响因子：3.5； 五年影响因子：4.1 ）

ISSN： 1471-2164

年卷期： 2024 年 25 卷 1 期

页码：

收录情况： SCI

摘要： BackgroundElucidating the genetic variation underlying phenotypic diversity will facilitate improving production performance in livestock species. The Tibetan sheep breed in China holds significant historical importance, serving as a fundamental pillar of Qinghai's animal husbandry sector. The Plateau-type Tibetan sheep, comprising 90% of the province's population, are characterized by their tall stature and serve as the primary breed among Tibetan sheep. In contrast, Zhashijia sheep exhibit larger size and superior meat quality. These two species provide an excellent model for elucidating the genetic basis of body size variation. Therefore, this study aims to conduct a comprehensive genome-wide association study on these two Tibetan sheep breeds to identify single nucleotide polymorphism loci and regulatory genes that influence body size traits in Tibetan sheep.ResultIn this study, the phenotypic traits of body weight, body length, body height, chest circumference, chest depth, chest width, waist angle width, and pipe circumference were evaluated in two Tibetan sheep breeds: Plateau-type sheep and Zhashijia Tibetan sheep. Whole genome sequencing generated 48,215,130 high-quality SNPs for genome-wide association study. Four methods were applied and identified 623 SNPs significantly associated with body size traits. The significantly associated single nucleotide polymorphisms identified in this study are located near or within 111 candidate genes. These genes exhibit enrichment in the cAMP and Rap1 signaling pathways, significantly affecting animal growth, and body size. Specifically, the following genes were associated: ASAP1, CDK6, FRYL, NAV2, PTPRM, GPC6, PTPRG, KANK1, NTRK2 and ADCY8.ConclusionBy genome-wide association study, we identified 16 SNPs and 10 candidate genes associated with body size traits in Tibetan sheep, which hold potential for application in genomic selection breeding programs in sheep. Identifying these candidate genes will establish a solid foundation for applying molecular marker-assisted selection in sheep breeding and improve our understanding of body size control in farmed animals.