文献类型： 外文期刊

作者： Liu, Xinyong ¹ ; Zhang, Xichun ¹ ; Cao, Ruijie ¹ ; Jiao, Guiai ¹ ; Hu, Shikai ¹ ; Shao, Gaoneng ¹ ; Sheng, Zhonghua ¹ ; X ¹ ;

作者机构： 1.China Natl Rice Res Inst, China Natl Ctr Rice Improvement, State Key Lab Rice Biol, Hangzhou 310006, Peoples R China

2.Guizhou Acad Agr Sci, Guizhou Rice Res Inst, Guiyang 550006, Peoples R China

关键词： CDE4; chloroplast development; pentatricopeptide repeat (PPR) protein; RNA splicing; low-temperature; Oryza sativa L

期刊名称：JOURNAL OF INTEGRATIVE PLANT BIOLOGY （ 影响因子：7.061； 五年影响因子：6.002 ）

ISSN： 1672-9072

年卷期： 2021 年 63 卷 10 期

页码：

收录情况： SCI

摘要： Pentatricopeptide repeat (PPR) proteins play important roles in the post-transcriptional modification of organellar RNAs in plants. However, the function of most PPR proteins remains unknown. Here, we characterized the rice (Oryza sativa L.) chlorophyll deficient 4 (cde4) mutant which exhibits an albino phenotype during early leaf development, with decreased chlorophyll contents and abnormal chloroplasts at low-temperature (20 degrees C). Positional cloning revealed that CDE4 encodes a P-type PPR protein localized in chloroplasts. In the cde4 mutant, plastid-encoded polymerase (PEP)-dependent transcript levels were significantly reduced, but transcript levels of nuclear-encoded genes were increased compared to wild-type plants at 20 degrees C. CDE4 directly binds to the transcripts of the chloroplast genes rpl2, ndhA, and ndhB. Intron splicing of these transcripts was defective in the cde4 mutant at 20 degrees C, but was normal at 32 degrees C. Moreover, CDE4 interacts with the guanylate kinase VIRESCENT 2 (V2); overexpression of V2 enhanced CDE4 protein stability, thereby rescuing the cde4 phenotype at 20 degrees C. Our results suggest that CDE4 participates in plastid RNA splicing and plays an important role in rice chloroplast development under low-temperature conditions.