Changes in gut microbiota affect DNA methylation levels and development of chicken muscle tissue
文献类型： 外文期刊
作者： Xu, Yibin 1 ; Huang, Yunpeng 1 ; Wei, Shenghua 1 ; Tian, Jinghong 1 ; Huang, Yulin 5 ; Nie, Qinghua 1 ; Zhang, Dexiang 1 ;
作者机构： 1.South China Agr Univ, State Key Lab Livestock & Poultry Breeding, Guangzhou 510642, Peoples R China
2.South China Agr Univ, Lingnan Guangdong Lab Agr, Guangzhou 510642, Peoples R China
3.South China Agr Univ, Guangzhou Prov Key Lab Agroanim Genom & Mol Breedi, Minist Agr & Rural Affair, Guangzhou 510642, Peoples R China
4.South China Agr Univ, Key Lab Chicken Genet Breeding & Reprod, Minist Agr & Rural Affair, Guangzhou 510642, Peoples R China
5.Wens Foodstuff Grp Co Ltd, Yunfu 527400, Peoples R China
关键词： Gut microbiome; Yellow-feathered chicken; Growth and development; DNA methylation
期刊名称：POULTRY SCIENCE （ 影响因子：4.2； 五年影响因子：4.5 ）
ISSN： 0032-5791
年卷期： 2025 年 104 卷 3 期
页码：
收录情况： SCI
摘要： The intestinal microbiome is essential in regulating host muscle growth and development. Antibiotic treatment is commonly used to model dysbiosis of the intestinal microbiota, yet limited research addresses the relationship between gut microbes and muscle growth in yellow-feathered broilers. In this study, Xinghua chickens were administered broad-spectrum antibiotics for eight weeks to induce gut microbiome suppression. We investigated the relationships between the gut microbiome and muscle growth using 16S rRNA sequencing and transcriptomic analysis. Results indicated that antibiotic treatment significantly reduced body weight, dressed weight, eviscerated weight, and breast and leg muscle weight. Microbial diversity and richness in the duodenum, jejunum, ileum, and cecum were significantly decreased. The relative abundances of Firmicutes, Actinobacteria, and Bacteroidetes declined, while Proteobacteria increased. This microbial imbalance led to 298 differentially expressed genes (DEGs) in muscle tissue, of which 67 down-regulated genes were enriched in skeletal muscle development, including MYF6, MYBPC1 and METTL21C genes essential for muscle development. The DEGs were primarily involved in the MAPK signaling pathway, calcium signaling pathway, ECM-receptor interaction, actin cytoskeleton regulation, and nitrogen metabolism. Correlation analysis showed that dysregulation of the cecal microbiome had the most substantial effect on muscle growth and development. Furthermore, intestinal microbiome dysregulation reduced DNMT3b and METTL21C mRNA expression in muscle tissue, lowered overall DNA methylation and SAM levels, and induced methylation changes that impacted skeletal muscle development. This study demonstrates that gut microbiota influence DNA methylation in muscle tissue, thereby associated with muscle growth and development.
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