您好，欢迎访问湖北省农业科学院 机构知识库！
湖北省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Target silencing of porcine SPAG6 and PPP1CC by shRNA attenuated sperm motility

文献类型： 外文期刊

作者： Ren, Hongyan 1 ; Zhang, Yandi 1 ; Bi, Yanzhen 1 ; Wang, Heng 2 ; Fang, Guijie 3 ; Zhao, Pengxiang 2 ;

作者机构： 1.Hubei Acad Agr Sci, Hubei Key Lab Anim Embryo Engn & Mol Breeding, Wuhan, Hubei, Peoples R China

2.Shandong Agr Univ, Coll Anim Sci & Technol, Tai An, Peoples R China

3.Hubei Univ Technol, Coll Bioengn, Minist Educ, Key Lab Fermentat Engn, Wuhan, Hubei, Peoples R China

4.61 Daizong St, Tai An, Shandong, Peoples R China

5.28 Nanli Rd, Wuhan, Hubei, Peoples R China

关键词： SPAG6; PPP1CC; Sperm quality; Pig

期刊名称：THERIOGENOLOGY （ 影响因子：2.8； 五年影响因子：2.6 ）

ISSN： 0093-691X

年卷期： 2024 年 219 卷

页码：

收录情况： SCI

摘要： The quality of sperm significantly influences the reproductive efficiency of pig herds. High-quality sperm is necessary for efficient fertilization and to maximize the litter numbers in commercial pig farming. However, the understanding of genes regulating porcine sperm motility and viability is limited. In this study, we validated porcine sperm/Sertoli-specific promoters through the luciferase reporter system and identified vital genes for sperm quality via loss-of-function means. Further, the shRNAs driven by the ACE and SP-10 promoters were used to knockdown the SPAG6 and PPP1CC genes which were provisionally important for sperm quality. We assessed the effects of SPAG6 and PPP1CC knockdown on sperm motility by using the sperm quality analyzer and flow cytometry. The results showed that the ACE promoter is active in both porcine Sertoli cells and sperms, whereas the SP-10 promoter is operating exclusively in sperm cells. Targeted interference with SPAG6 and PPP1CC expression in sperm cells decreases the motility and increases apoptosis rates in porcine sperms. These findings not only offer new genetic tools for targeting male germ cells but also highlight the crucial roles of SPAG6 and PPP1CC in porcine sperm function.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：湖北省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台