Transcriptome analysis of the effect of high-temperature on nutrient metabolism in juvenile grass carp (Ctenopharyngodon idellus)
文献类型： 外文期刊
作者： Huang, Dongyu 1 ; Ren, Mingchun 1 ; Liang, Hualiang 2 ; Ge, Xianping 1 ; Xu, Hao 1 ; Wu, Longhua 1 ;
作者机构： 1.Nanjing Agr Univ, Wuxi Fisheries Coll, Wuxi 214081, Jiangsu, Peoples R China
2.Chinese Acad Fishery Sci CAFS, Freshwater Fisheries Res Ctr FFRC, Key Lab Genet Breeding Aquat Anim & Aquaculture B, Wuxi 214081, Jiangsu, Peoples R China
关键词： Juvenile grass carp(Ctenopharyngodonidellus); Transcriptome analysis; High temperature; Nutrient metabolism
期刊名称：GENE （ 影响因子：3.913； 五年影响因子：3.48 ）
ISSN： 0378-1119
年卷期： 2022 年 809 卷
页码：
收录情况： SCI
摘要： To investigate the variations in gene expression in grass carp under high-temperature stress, two libraries were constructed from a high-temperature treatment group (T33) and a control group (T27) and sequenced using Illumina sequencing technology. The results showed that sequencing generated a total of 279,398,348 raw reads, approximately 40.7-51.8 M clean reads were obtained from each library, and the percentage of uniquely mapped transcripts ranged from 80.13 to 84.58%. A total of 260 differentially expressed genes (DEGs) were identified under high-temperature stress, among which 84 genes were upregulated and 176 genes were downregulated. Ten DEGs were randomly selected for quantitative RT-PCR (qRT-PCR) analysis, and the results confirmed that the transcriptome analysis was reliable. Furthermore, the DEGs were subjected to Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis, and the results showed that most of the DEGs were involved in protein, lipid and carbohydrate metabolism. Moreover, plasma urea nitrogen (Urea) and tri-glyceride (TG) contents were significantly lower in the high-temperature treatment group than in the control group (P < 0.01). In summary, these results indicated that high-temperature stress could inhibit protein syn -thesis, decrease fatty acid synthesis, and weaken carbohydrate metabolism in juvenile grass carp.
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