Metabonomics analysis of flavonoids in seeds and sprouts of two Chinese soybean cultivars
文献类型: 外文期刊
作者: Bi, Weiwei 1 ; Zhao, Guixing 2 ; Zhou, Yutong 4 ; Xia, Xiaoyu 2 ; Wang, Jinsheng 2 ; Wang, Guangjin 2 ; Lu, Shuwen 5 ; He, Wenjin 2 ; Bi, Taifei 3 ; Li, Jinrong 2 ;
作者机构: 1.Heilongjiang Acad Agr Sci, Postdoctoral Stn, Harbin 150086, Peoples R China
2.Heilongjiang Acad Agr Sci, Soybean Res Inst, Harbin 150086, Peoples R China
3.Yulin Univ, Coll Life Sci, Yulin 719000, Peoples R China
4.Heilongjiang Univ, Sch Hydraul & Elect Power, Harbin 150086, Peoples R China
5.Heilongjiang Acad Agr Sci, Food Proc Res Inst, Harbin 150086, Peoples R China
期刊名称:SCIENTIFIC REPORTS ( 影响因子:4.996; 五年影响因子:5.516 )
ISSN: 2045-2322
年卷期: 2022 年 12 卷 1 期
页码:
收录情况: SCI
摘要: A popular food in China, soybean seeds and sprouts contained many biologically active substances which are beneficial to the human body, such as flavonoids. Northeast of China is the main producing area of soybean. The experimental materials came from the main soybean producing areas in Northeast China, this study compared flavonoids of two China cultivars of soybeans, Heinong52(HN52) and Heinong71(HN71). Here, we also considered the effects of germination on the chemical profile of flavonoids. Using a LC-ESI-MS/MS system, 114 differential flavonoid metabolites were identified. A total of 18 metabolites were significantly different between the two soybean varieties before germination, of which 14 were up-regulated and 4 were down-regulated. After germination, 33 significantly different metabolites were found in the two soybean sprouts, of which 19 were up-regulated and 14 were down-regulated. These experimental results revealed significant up-regulation of metabolites in soybean sprouts compared with soybean seeds, thus suggesting that soybean germination may increase content of flavonoid metabolites. There are six main pathways for the synthesis of flavonoids: isoflavonoid biosynthesis, flavonoid biosynthesis, flavone and flavonol biosynthesis, biosynthesis of secondary metabolites, and biosynthesis of phenylpropanoids. Soybean seeds lack flavone and flavanol biosynthesis and develop the capacity for this biosynthetic pathway after germination as sprouts. Isoflavonoid biosynthesis is the most abundantly utilized pathway.
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