文献类型： 外文期刊

作者： Luo, Yin ³ ; Qin, Cheng ¹ ; Qiu, Huarong ¹ ; Zhang, Xiaowei ¹ ; Tang, Xiangqun ¹ ; Luo, Xirong ¹ ; Luo, Yong ¹ ; Yang, Hong ² ; Chen, Xiaocui ¹ ;

作者机构： 1.Zunyi Acad Agr Sci, Zunyi 563006, Guizhou, Peoples R China

2.Guizhou Acad Agr Sci, Guiyang 550025, Guizhou, Peoples R China

3.Hunan Univ, Coll Biol, Longping Branch, Changsha 410125, Hunan, Peoples R China

4.Zunyi Vocat & Tech Coll, Modern Agr Dept, Zunyi 563000, Guizhou, Peoples R China

关键词： Capsicum annuum; miRNA; Cucumber mosaic virus; Small RNA sequencing; Stem-loop RT-PCR

期刊名称：PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY （ 影响因子：2.7； 五年影响因子：2.9 ）

ISSN： 0885-5765

年卷期： 2023 年 124 卷

页码：

收录情况： SCI

摘要： MicroRNAs (miRNAs) are a category of short, highly conserved, noncoding RNAs that modulate various phys-iological activities correlated with plant growth, stress response, and virus-host interaction. However, the miRNAs associated with cucumber mosaic virus (CMV) resistance in hot pepper (Capsicum annuum L.) remain unexplored. The present work employed small RNA sequencing to compare CMV-infected pepper seedlings with the control seedlings at different time points. The analysis using miRDeep2 software identified 216 known and 34 novel miRNAs from the 12 pepper libraries generated in this study. Further research predicted 3914 targets for these novel miRNAs, of which 3502 were conserved miRNA target genes and 412 were novel miRNA target genes. Functional analysis revealed significant enrichment of these miRNA in 'plant-pathogen interaction', 'ubiquitin-mediated proteolysis', and 'metabolic pathways'. Finally, quantitative RT-PCR validated the sRNA sequencing data. These findings set the groundwork for further studies on the CMV resistance mechanisms of pepper.