Application of capillary electrophoresis in enzyme inhibitors screening from natural products
文献类型： 外文期刊
作者： Li Feng 1 ; Zhang Yanmei 2 ; Kang Jingwu 3 ;
作者机构： 1.Xian Univ, Sch Chem Engn, Xian 710065, Peoples R China
2.Shanghai Acad Agr Sci, Inst Agrifood Stand & Testing Technol, Shanghai 201403, Peoples R China
3.Chinese Acad Sci, State Key Lab Bioorgan & Nat Prod Chem, Shanghai Inst Organ Chem, Shanghai 200032, Peoples R China
关键词： capillary electrophoresis (CE); drug screening; enzyme inhibitors; natural products
期刊名称：CHINESE JOURNAL OF CHROMATOGRAPHY （ 影响因子：0.7； 五年影响因子：0.5 ）
ISSN： 1000-8713
年卷期： 2020 年 38 卷 5 期
页码：
收录情况： SCI
摘要： As aberrant enzyme functions are often closely associated with various diseases, enzymes represent one of the most important drug target groups. Historically, natural products have been the most productive sources for drug discovery. However, the complexity of the components leads to a bottleneck in separation and purification, thus restricting activity. based drug screening. Due to the unique advantages such as high separation efficiency and without sample matrix interference, the advantage of capillary electrophoresis (CE) directly applied to extracts without purification for activity. based drug screening is of great attractive. In this review, we have summarized the application of CE for screening enzyme inhibitors in natural products during the last decade. In particular, we discuss important examples for screening inhibitors of transferases (kinases), hydrolases, oxidoreductases, etc. We also present an overview of the technical details concerning the separation modes in CE and enzyme kinetics. Finally, we provide future prospects for the application of CE to the screening of active ingredients in natural products.
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