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miR-PC-3p-241582_34 Contributes to the Infection of Athetis lepigone by Regulating the Expression of HSWP4 in Nosema bombycis

文献类型： 外文期刊

作者： Zhang, Haijian 1 ; Sun, Xuelian 1 ; Xiao, Hongli 1 ; Liu, Shusen 1 ; Guo, Ning 1 ; Li, Yaofa 1 ; Shi, Jie 1 ;

作者机构： 1.Hebei Acad Agr & Forestry Sci, Inst Plant Protect, Key Lab Integrated Pest Management Crops Northern, Minist Agr & Rural Affairs, Baoding 071000, Hebei, Peoples R China

2.IPM Innovat Ctr Hebei Prov, Int Sci & Technol Joint Res Ctr IPM Hebei Prov, Baoding 071000, Hebei, Peoples R China

关键词： microsporidia; spore wall protein; regulatoryrelationship; microRNA

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.2； 五年影响因子：6.4 ）

ISSN： 0021-8561

年卷期： 2024 年 72 卷 48 期

页码：

收录情况： SCI

摘要： Athetis lepigone is a recurring pest in the maize seedling stage under the wheat-maize no-tillage direct seeding system in China's summer maize region. Our previous research identified a highly pathogenic Nosema bombycis to A. lepigone, which spore wall protein plays an important role in the infection process. However, the regulatory mechanism of this spore wall protein is still unclear. In this study, we explored the regulatory mechanism of miRNAs on spore wall proteins. Transcriptome sequencing results showed that expression of the spore wall protein, HSWP4, significantly increased in the germination group compared to dormancy group. Silencing of HSWP4 reduced the number of microsporidian spores breaking through the midgut wall cells of A. lepigone. Association analysis of small RNA and mRNA revealed that the targeting site of miR-PC-3p-241582_34 on HSWP4 was located in the CDS region, and miR-PC-3p-241582_34 had a significant negative regulatory relationship with HSWP4. The dual luciferase reporter assay demonstrated that miR-PC-3p-241582_34 significantly affected the luciferase activity of the HSWP4-3 ' UTR expression vector (P < 0.05). Delivery of miRNA mimics decreased the expression of HSWP4 and inhibited the behavior of microsporidian spores breaking through the midgut wall of A. lepigone. On the other hand, delivery of inhibitors produced opposite results, indicating that the miR-HSWP4 pathway plays an important role in microsporidian infection of A. lepigone. This study provides a new theoretical basis for understanding the pathogenic mechanism and gene regulation of microsporidia, as well as for the green control of A. lepigone.

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