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Activated Expression of Rice DMR6-like Gene OsS3H Partially Explores the Susceptibility to Bacterial Leaf Streak Mediated by Knock-Out OsF3H04g

文献类型： 外文期刊

作者： Wu, Tao 1 ; Bi, Yunya 2 ; Yu, Yue 2 ; Zhou, Zhou 2 ; Yuan, Bin 4 ; Ding, Xinhua 5 ; Zhang, Qingxia 1 ; Chen, Xiangsong 2 ; Yang, Hong 3 ; Liu, Haifeng 5 ; Chu, Zhaohui 2 ;

作者机构： 1.Yangzhou Univ, Coll Plant Protect, Yangzhou 225009, Peoples R China

2.Wuhan Univ, Coll Life Sci, State Key Lab Hybrid Rice, Hubei Hongshan Lab, Wuhan 430072, Peoples R China

3.Hubei Univ, Sch Life Sci, State Key Lab Biocatalysis & Enzyme Engn, Wuhan 430062, Peoples R China

4.Hubei Acad Agr Sci, Inst Plant Protect & Soil Fertilizer, Wuhan 430064, Peoples R China

5.Shandong Agr Univ, State Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China

关键词： rice; bacterial leaf streak; DMR6-like; susceptibility; salicylic acid

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.6； 五年影响因子：6.2 ）

ISSN： 1661-6596

年卷期： 2023 年 24 卷 17 期

页码：

收录情况： SCI

摘要： Downy Mildew Resistance 6-like (DMR6-like) genes are identified as salicylic acid (SA) hydroxylases and negative regulators of plant immunity. Previously, we identified two rice DMR6-like genes, OsF3H03g, and OsF3H04g, that act as susceptible targets of transcription activator-like effectors (TALEs) from Xanthomonas oryzae pv. oryzicola (Xoc), which causes bacterial leaf streak (BLS) in rice. Furthermore, all four homologs of rice DMR6-like proteins were identified to predominantly carry the enzyme activity of SA 5-hydroxylase (S5H), negatively regulate rice broad-spectrum resistance, and cause the loss of function of these OsDMR6s, leading to increased resistance to rice blast and bacterial blight (BB). Here, we curiously found that an OsF3H04g knock-out mutant created by T-DNA insertion, osf3h04g, was remarkedly susceptible to BLS and BB and showed an extreme reduction in SA content. OsF3H04g knock-out rice lines produced by gene-editing were mildly susceptible to BLS and reduced content of SA. To explore the susceptibility mechanism in OsF3H04g loss-of-function rice lines, transcriptome sequencing revealed that another homolog, OsS3H, had induced expression in the loss-of-function OsF3H04g rice lines. Furthermore, we confirmed that a great induction of OsS3H downstream and genomically adjacent to OsF3H04g in osf3h04g was primarily related to the inserted T-DNA carrying quadruple enhancer elements of 35S, while a slight induction was caused by an unknown mechanism in gene-editing lines. Then, we found that the overexpression of OsS3H increased rice susceptibility to BLS, while gene-editing mediated the loss-of-function OsS3H enhanced rice resistance to BLS. However, the knock-out of both OsF3H04g and OsS3H by gene-editing only neutralized rice resistance to BLS. Thus, we concluded that the knock-out of OsF3H04g activated the expression of the OsS3H, partially participating in the susceptibility to BLS in rice.

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