文献类型： 外文期刊
作者： Zhang, Xuelian 1 ; Zhang, Mengyang 1 ; Wang, Chunzhi 1 ; Song, Qingchuan 1 ; Yang, Haiyan 1 ; Tang, Qi 1 ; Zhao, Qiaoling 2 ; Wang, Jing 3 ; Pan, Chuanying 1 ;
作者机构： 1.Northwest A&F Univ, Coll Anim Sci & Technol, Key Lab Anim Genet Breeding & Reprod Shaanxi Prov, Yangling 712100, Peoples R China
2.Jiangsu Univ Sci & Technol, Sch Biotechnol, Jiangsu Key Lab Sericultural Biol & Biotechnol, Zhenjiang 212100, Peoples R China
3.Henan Acad Agr Sci, Inst Anim Husb, Henan Pig Breeding Engn Res Ctr, Henan Key Lab Farm Anim Breeding & Nutr Regulat, Zhengzhou 450002, Peoples R China
关键词：
Chlorpyrifos; male infertility; RNA-Sequencing; PI3K-AKT;
期刊名称：CELLS （ 影响因子：5.2； 五年影响因子：6.1 ）
ISSN：
年卷期： 2025 年 14 卷 13 期
页码：
收录情况： SCI
摘要： Chlorpyrifos (CPF) has been extensively utilized in recent decades due to its highly efficient insecticidal properties. However, the widespread use of pesticides has posed new challenges to male reproduction. This study aims to explore the potential molecular mechanisms of male reproductive decline induced by CPF. We employ flow cytometry, qRT-PCR, Western blot, RNA sequencing, and bioinformatics analysis to investigate the potential molecular mechanisms involved in CPF-induced male reproductive damage in GC2spd cells. Our results revealed that after 24 h of CPF treatment, the cell viability, cell cycle, apoptosis, and reactive oxygen species (ROS) accumulation of GC2spd cells were significantly affected in vitro. RNA sequencing analysis data indicated that a total of 626 genes were differentially expressed compared to the DMSO group, especially for Efcab6, Nox3, and Cmpk2. These differential genes were mainly enriched in signaling pathways such as PI3K-AKT and glutamine metabolism. In addition, further validation through qRT-PCR, Western blot, and experiments involving the inhibition of intracellular ROS generation with N-acetylcysteine collectively confirmed that CPF induces male reproductive damage through the ROS/AKT/Efcab6 pathway. These studies elucidate potential targets and molecular mechanisms underlying CPF-induced male infertility, providing a theoretical basis for the prevention of male reproductive damage caused by pesticide residues.
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