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Identification of Solanum lycopersicum L. Casein Kinase I-like Gene Family and Analysis of Abiotic Stress Response

文献类型： 外文期刊

作者： Jia, Miao 1 ; Xie, Xiaoxiao 2 ; Wang, Quanhua 1 ; Wang, Xiaoli 1 ; Zhang, Yingying 2 ;

作者机构： 1.Shanghai Normal Univ, Coll Life Sci, Shanghai 201400, Peoples R China

2.Shanghai Acad Agr Sci, Protected Hort Inst, Shanghai Key Lab Protected Hort Technol, Shanghai 201403, Peoples R China

关键词： tomato (Solanum lycopersicum); CKL gene; bioinformatics analysis; abiotic stress

期刊名称：GENES （ 影响因子：2.8； 五年影响因子：3.2 ）

ISSN：

年卷期： 2025 年 16 卷 7 期

页码：

收录情况： SCI

摘要： Background: Casein kinase I-like (CKL) protein is a member of the serine/threonine kinase CKI family and plays a pivotal regulatory role in various eukaryotic cellular processes, including stress responses. Objectives: This study aims to systematically identify the CKL gene family in the tomato genome and investigate its responsiveness to abiotic stress. Methods: Members of SlCKL were identified through genome-wide bioinformatics analysis, and their physicochemical properties, chromosomal localization, gene structure, conserved domains, phylogenetic relationships, cis-acting elements, cross-species collinearity, and tissue expression profiles were comprehensively analyzed. The expression patterns of SlCKL genes under abiotic stress were validated using real-time quantitative PCR. Results: A total of 16 SlCKL genes were identified and classified into three subfamilies (I-III), which are unevenly distributed across nine chromosomes, predominantly clustered at the ends. The gene structure, motifs, and functional domains exhibit high conservation. Collinearity analysis revealed stronger synteny between tomato and Arabidopsis thaliana or pepper compared to rice, maize, or tobacco, suggesting a common ancestral origin. The tissue expression profile indicates that SlCKLs are preferentially transcribed in roots. Promoter analysis and qRT-PCR validation demonstrated differential responses of SlCKLs to various abiotic stresses, such as drought, salt, heat, cold, and ABA treatment. Conclusions: This study represents the first systematic identification of the tomato SlCKL gene family, elucidating its evolutionary relationships, structural characteristics, tissue-specific expression patterns, and differential responsiveness to abiotic stress, thereby providing a critical foundation for further investigation into the molecular mechanisms underlying CKL-mediated abiotic stress adaptation in tomatoes.

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