文献类型： 外文期刊

作者： Zhang, Fengfeng ¹ ; Zhou, Ke ¹ ; Xie, Fengxing ¹ ; Zhao, Qiong ¹ ;

作者机构： 1.Tianjin Acad Agr Sci, Inst Agr Resources & Environm, Tianjin 300384, Peoples R China

关键词： Lactic acid bacteria; Antimicrobial abilities; Agar diffusion method; Pathogenic bacteria; Aquaculture

期刊名称：ARCHIVES OF MICROBIOLOGY （ 影响因子：2.667； 五年影响因子：2.729 ）

ISSN： 0302-8933

年卷期： 2022 年 204 卷 12 期

页码：

收录情况： SCI

摘要： This study aimed to evaluate the antibacterial properties of nine lactic acid bacterial strains. The agar diffusion method (Oxford cup method) was used to assess the antimicrobial activity against pathogenic bacteria in aquaculture. The results showed that all selected strains inhibited the growth of Photobacterium damsel, Vibrio alginolyticus, Listonella anguillarum, Edwardsiella ictaluri, Aeromonas hydrophila, V. arahaemolyticus, Edwardsiella tarda and V. harveyi, but did not inhibit the growth of Metschnikowia bicuspidata. Among these strains of lactic acid bacteria, r1 was selected for its obvious antibacterial activity against eight kinds of pathogenic bacteria by 96-well plate method. Its inhibitory rate reached 96%, and it could inhibit the growth of six pathogenic bacteria at 121 celcius, 20 min and pH 3.5-4.0. It was identified as Lactiplantibacillus plantarum by morphological observation and 16S rRNA sequencing analysis. Because strain r1 was isolated from culture ponds and exerted strong inhibitory effects on pathogenic bacteria, it holds potential as an agent to prevent and control infectious diseases in aquaculture. This study provides a foundation for the development and utilization of probiotics in aquaculture.