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Boronic acid-functionalized Fe3O4 nanoparticles for activity-preserved enrichment of low-abundance bacteria from real samples

文献类型： 外文期刊

作者： Chen, Jingwen 1 ; Li, Shaobo 1 ; Deng, Bin 1 ; Wang, Hongyuan 1 ; Sun, Wenkui 3 ; Li, Li 2 ; Bai, Zongchun 2 ; Liu, Jing 1 ;

作者机构： 1.China Pharmaceut Univ, Sch Pharm, 24 Tongjiaxiang Rd, Nanjing 210009, Peoples R China

2.Minist Agr & Rural Affairs, Inst Agr Facil & Equipment, Jiangsu Acad Agr Sci, Key Lab Protected Agr Engn Middle & Lower Reaches, 50 Zhongling St, Nanjing 210014, Peoples R China

3.Nanjing Med Univ, Dept Resp & Crit Care Med, Affiliated Hosp 1, 300 Guangzhou Rd, Nanjing 210029, Peoples R China

期刊名称：RSC ADVANCES （ 影响因子：4.6； 五年影响因子：4.3 ）

ISSN：

年卷期： 2025 年 15 卷 7 期

页码：

收录情况： SCI

摘要： Pathogenic bacterial infections represent a significant and ongoing threat to public health. The development of a sensitive, convenient, and accurate method for diagnosing pathogenic bacteria is a formidable challenge due to their low abundance in complex biological samples, especially in the early stages of diseases. In this study, a kind of phenylboronic acid-functionalized Fe3O4 nanoparticles (NPs), known as Fe3O4@poly(PEGDA-co-MAAPBA) NPs, was developed for effectively enriching low levels of pathogenic bacteria from complex samples and then diagnosing them through microbiological cultures. In this design, the resultant Fe3O4@poly(PEGDA-co-MAAPBA) NPs could recognize pathogenic bacteria because of the reversible reactions between the phenylboronic acid groups on the NPs and the cis-diol structures outside of the bacterial cells. By exploiting the magnetic properties of Fe3O4 NPs, bacteria were able to anchored onto the resulting NPs (NPs@bacteria) for easy enrichment. Utilizing microbiological culture techniques, successful cultivation of NPs@bacteria was achieved, demonstrating that bacterial activity remained unaffected during the enrichment process. The proposed method exhibited a limit of detection as low as 0.4 colony-forming units per milliliter. The Fe3O4@poly(PEGDA-co-MAAPBA) NPs were applied successfully for testing Staphylococcus aureus in urine samples which were typically considered to be free of bacterial contamination, indicating excellent selectivity and enrichment capability of the prepared NPs in complex samples. It suggests that the Fe3O4@poly(PEGDA-co-MAAPBA) NPs have the potential to become a powerful tool for early diagnosis of pathogenic bacteria in the clinic.

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