Analysis of expression characteristics of soybean leaf and root tissue-specific promoters in Arabidopsis and soybean
文献类型： 外文期刊
作者： Xun, Hongwei 1 ; Zhang, Xue 2 ; Yu, Jiamiao 2 ; Pang, Jinsong 2 ; Wang, Shucai 3 ; Liu, Bao 2 ; Dong, Yingshan 1 ; Jiang, 1 ;
作者机构： 1.Jilin Acad Agr Sci, Jilin Prov Key Lab Agr Biotechnol, Changchun 130033, Peoples R China
2.Northeast Normal Univ, Key Lab Mol Epigenet MOE, Changchun 130024, Peoples R China
3.Linyi Univ, Sch Life Sci, Lab Plant Mol Genet & Crop Gene Editing, Linyi 276000, Shandong, Peoples R China
关键词： Soybean; Tissue-specific promoter; GUS staining; Salt resistance; Arabidopsis thaliana
期刊名称：TRANSGENIC RESEARCH （ 影响因子：2.788； 五年影响因子：2.377 ）
ISSN： 0962-8819
年卷期： 2021 年 30 卷 6 期
页码：
收录情况： SCI
摘要： The characterization of tissue-specific promoters is critical for studying the functions of genes in a given tissue/organ. To study tissue-specific promoters in soybean, we screened tissue-specific expressed genes using published soybean RNA-Seq-based transcriptome data coupled with RT-PCR analysis. We cloned the promoters of three genes, GmADR1, GmBTP1, and GmGER1, and constructed their corresponding beta-Glucuronidase (GUS) promoter-GUS reporter vectors. We generated transgenic Arabidopsis plants and examined the expression patterns of these promoters by GUS staining and RT-PCR analysis. We also transformed the promoter-GUS reporter vectors into soybean to obtain hairy roots, and examined promoter expression by GUS staining. We found a root-specific expression pattern of GmADR1 and GmBTP1 in both Arabidopsis and soybean, and the promoter of GmGER1 showed a leaf-specific pattern in transgenic Arabidopsis plants. To test the potential utility of these promoters in soybean improvement by transgenic means, we used the GmADR1 promoter to drive expression of a salt resistance gene in soybean, GmCaM4, by generating transgenic soybean plants. We found that the transgenic plants had significantly enhanced salt tolerance compared to non-transformed wild-type, suggesting that introducing endogenous promoters by transgenic means can drive the expression of functional genes in specific tissues and organs in soybean.
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