Identification of the microorganisms for methane-dependent arsenate reduction in wetland using DNA-stable isotope probing and metagenomics
文献类型： 外文期刊
作者： Yu, Xiaoxiao 1 ; Li, Jibing 2 ; Zhou, Yujie 1 ; Chen, Yun 1 ; Zou, Lina 4 ; Luo, Chunling 2 ; Shen, Chaofeng 5 ; Liu, Fengjie 6 ; Xu, Jianming 1 ; Tang, Xianjin 1 ;
作者机构： 1.Zhejiang Univ, Coll Environm & Resource Sci, State Key Lab Soil Pollut Control & Safety, Hangzhou 310058, Peoples R China
2.Chinese Acad Sci, Guangzhou Inst Geochem, State Key Lab Organ Geochem, Guangzhou 510640, Peoples R China
3.Chinese Acad Sci, Guangzhou Inst Geochem, Guangdong Hong Kong Macao Joint Lab Environm Pollu, Guangzhou 510640, Peoples R China
4.Zhejiang Acad Agr Sci, Zhejiang Inst Landscape Plants & Flowers, Hangzhou 311251, Peoples R China
5.Zhejiang Univ, Coll Environm & Resource Sci, Dept Environm Engn, Hangzhou 310058, Peoples R China
6.Imperial Coll London, Grantham Inst Climate Change & Environm, London SW7 2AZ, England
关键词： Methane oxidation; Arsenate reduction; DNA-SIP; Metagenomic; Wetland
期刊名称：WATER RESEARCH （ 影响因子：12.4； 五年影响因子：12.9 ）
ISSN： 0043-1354
年卷期： 2025 年 284 卷
页码：
收录情况： SCI
摘要： The methane-dependent arsenate reduction (M-AsR) process is recently demonstrated to enhance the release of mobile and toxic arsenite [As(III)], with critical implications for ecosystem safety in wetland ecosystem. However, the key functional microorganisms and the underlying metabolic mechanisms of M-AsR in wetland remain unclear. In this study, 13C-labeled methane (CH4) was used as the sole carbon source to track the active microorganisms responsible for M-AsR. DNA-stable isotope probing (DNA-SIP) combined with amplicon and metagenomic sequencing was further employed to identify the microorganisms involved in M-AsR. The results showed that arsenate [As(V)] reduction occurred exclusively in the treatment amended with both CH4 and As(V). After a 50-day incubation, significant shifts in the relative abundance of functional genes (pmoA, ANME-2d mcrA and arrA) were observed in the heavy DNA fractions from the treatment amended with 13CH4 and As(V), indicating the incorporation of 13C into M-AsR microorganisms. Furthermore, the Methanobacterium, Methylobacter and arsenate-reducing bacteria (Chryseobacterium and Hydrogenophaga) were the predominant genera in 13CH4As heavy fractions and identified as the potential microorganisms responsible for M-AsR in wetland. Metagenomic analysis further confirmed that most of these microorganisms contained genes for CH4 oxidation and As (V) reduction. This multi-omics approach provides mechanistic insights into microbial mediated As(V) reduction in methane-rich wetland area.
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