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The microsatellites and minisatellites in the genome of Fenneropenaeus chinensis

文献类型： 外文期刊

作者： Gao, H 1 ; Kong, J 2 ;

作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Minist Agr, Key Lab Sustainable Utilizat Marine Fisheries Res, Qingdao 266071, Peoples R China

2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Minist Agr, Key Lab Sustainable Utilizat Marine Fisheries Res, Qingdao 266071, Peoples R China; Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China; Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

关键词： microsatellites;minisatellites;F. chinensis;genome;repeat types;repeat classes;SIMPLE REPETITIVE DNA;TANDEM REPEATS;GENETIC DIVERSITY;MISMATCH REPAIR;SSR MARKERS;SEQUENCES;IDENTIFICATION;RECOMBINATION;DROSOPHILA;ABUNDANCE

期刊名称：DNA SEQUENCE （ 影响因子：0.408； 五年影响因子：0.594 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： Through two-time sequencing randomly in Fenneropenaeus chinensis , 2,597,000 bp cumulative length random genomic sequences about occupying 1.23% of the entire genome are obtained, in which the length of the first time sequencing is 8,84,000 bp, by cutting the genome DNA with Sau3AI enzyme, and the second is 1,713,000 bp by breaking the genome DNA with the physical method, ultrasonic. Using tandem repeat finder (TRF) soft to analyze the sequences, 4588 tandem repeats are found, in which the number of microsatellites (1-6 bp) is 3888, and 700 for minisatellites (>= 7 bp). The cumulative length of repeats is 3,05,555 bp, accounting for 11.72% of total cumulative sequence length, in which the cumulative length of microsatellites is 2,32,979 bp, accounting for 8.97% of total sequence length, and greater than those of other organisms, such as human and mosquito, etc. The dinucleotide repeat type is dominant in which the dominant repeat class is AT. The second abundant repeat type is trinucleotide, of which the dominant repeat class is AAT. Interestingly, of all of repeat types, the repeat numbers and repeat classes of primer number repeat types, such as pentanucleotide, heptanucleotide, elevennucleotide, etc. are less than those of repeat types beside them. The phenomena may involve the genesis and the evolution of microsatellites and minisatellites.

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