文献类型： 外文期刊

作者： Zhang, Dongyun ¹ ; Li, Shengjie ¹ ; Tian, Taihang ¹ ; Du, Jinxing ¹ ; Lei, Caixia ¹ ; Zhu, Tao ¹ ; Han, Linqiang ⁵ ; Song, Hongmei ¹ ;

作者机构： 1.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Key Lab Trop & Subtrop Fishery Resource Applicat &, Minist Agr & Rural Affairs, Guangzhou, Peoples R China

2.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Guangdong Prov Key Lab Aquat Anim Immunol & Sustai, Guangzhou, Peoples R China

3.Huzhou Univ, Coll Life Sci, Huzhou, Peoples R China

4.Shanghai Ocean Univ, Coll Fisheries Sci Educ, Shanghai, Peoples R China

5.Guangdong Prov Liangshi Aquaculture Seed Ind, Foshan, Peoples R China

关键词： MT; LE; growth; gonadal development; all-female largemouth bass

期刊名称：FRONTIERS IN PHYSIOLOGY （ 影响因子：3.4； 五年影响因子：4.0 ）

ISSN：

年卷期： 2024 年 15 卷

页码：

收录情况： SCI

摘要： In order to optimize the parameters for reversing masculinization and establish the techniques for sex induction of pseudo-males and creation of all-female fry in largemouth bass (Micropterus salmodies, LMB), 15-day-old LMB (1.00 +/- 0.10 cm in length, 0.10 +/- 0.01 g in weight) were fed a diet supplemented with either 17 alpha-methyltestosterone (MT) or letrozole (LE) and their combination. The experimental groups were M20 (20 mg/kg MT), L20 (20 mg/kg LE) and M10L10 (10 mg/kg MT and 10 mg/kg LE). The control group, named C, was not feed MT or LE. After 60 days, exogenous hormone in the diets was stopped and the effects of MT and LE on growth, male ratio, and gonadal development in LMB were evaluated. At 12-month-old, blood and gonadal tissue samples were collected to measure sex steroid hormones levels, analyze expression levels of dmrt1 and cyp19a1a genes, as well as examine the gonads morphology. The results showed no significant differences in growth between the experimental groups and the control group after a 60-day feeding period with the formulated diet (p > 0.05). The sex reversal ratio of M20, L20, M10L10 were 95.00%, 80.00%, 76.47%, respectively. The gonadal tissue sections showed that the gonadal structure of masculinized fish morphologic resembled that of control male fish. At 12-month-old, the sex reversal ratio in M20, L20, M10L10 and C groups were 100%, 86.67%, 73.33% and 50.00%, respectively. The testicular of pseudo-male fish in the M20 group exhibited well-developed morphology similarities to that of the control group males. However, the testes of pseudo-male fish in the L20 and M10L10 groups were smaller size Estradiol (E-2) levels in the experimental groups was significantly lower than those in the control group females (p < 0.05), while testosterone (T) levels were significantly higher than that of the control group (p < 0.05). Compared to the female fish in the control group, pseudo-male fish from all experimental groups showed significantly upregulated expression of dmrt1 (p < 0.05), and significantly downregulated expression of cyp19a1a (p < 0.05). Pseudo-males selected from group M20 exhibited a significantly higher proportion of female offspring (92.00%) compared to the control group (46.50%). In summary, 20 mg/kg MT was the optimal inducing concentration.