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An Improved Agrobacterium-Mediated Transformation Method for an Important Fresh Fruit: Kiwifruit (Actinidia deliciosa)

文献类型： 外文期刊

作者： Piao, Chun-Lan 1 ; Ding, Mengdou 1 ; Gao, Yongbin 1 ; Song, Tao 2 ; Zhu, Ying 2 ; Cui, Min-Long 1 ;

作者机构： 1.Zhejiang A&F Univ, Coll Hort Sci, Minist Agr & Rural Affairs, Key Lab Qual & Safety Control Subtrop Fruit & Vege, Hangzhou 311300, Peoples R China

2.Zhejiang Acad Agr Sci, Inst Virol & Biotechnol, State Key Lab Managing Biot & Chem Threats Qual &, Minist Agr & Rural Affairs,Key Lab Traceabil Agr G, Hangzhou 310021, Peoples R China

关键词： kiwifruit (Actinidia deliciosa); leaf explant; high-efficiency genetic transformation; Southern blot analysis; mature plant; stable GFP expression

期刊名称：PLANTS-BASEL （ 影响因子：4.1； 五年影响因子：4.5 ）

ISSN： 2223-7747

年卷期： 2025 年 14 卷 15 期

页码：

收录情况： SCI

摘要： Genetic transformation is an essential tool for investigating gene function and editing genomes. Kiwifruit, recognized as a significant global fresh fruit crop, holds considerable economic and nutritional importance. However, current genetic transformation techniques for kiwifruit are impeded by low efficiency, lengthy culture durations (a minimum of six months), and substantial labor requirements. In this research, we established an efficient system for shoot regeneration and the stable genetic transformation of the 'Hayward' cultivar, utilizing leaf explants in conjunction with two strains of Agrobacterium that harbor the expression vector pBI121-35S::GFP, which contains the green fluorescent protein (GFP) gene as a visible marker within the T-DNA region. Our results show that 93.3% of leaf explants responded positively to the regeneration medium, producing multiple independent adventitious shoots around the explants within a six-week period. Furthermore, over 71% of kanamycin-resistant plantlets exhibited robust GFP expression, and the entire transformation process was completed within four months of culture. Southern blot analysis confirmed the stable integration of GFP into the genome, while RT-PCR and fluorescence microscopy validated the sustained expression of GFP in mature plants. This efficient protocol for regeneration and transformation provides a solid foundation for micropropagation and the enhancement of desirable traits in kiwifruit through overexpression and gene silencing techniques.

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