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The Wx/SSIIa and GS3/GW7 Alleles, Both Individually and in Combination, Can Significantly Distinguish Rice Germplasm Quality

文献类型: 外文期刊

作者: Hao, Yuanyuan 1 ; Xu, Junfeng 2 ; Wu, Mingming 1 ; Zhu, Ying 3 ; Song, Jiayu 1 ; Han, Yifei 1 ; Li, Chunshou 1 ; Huang, Fudeng 1 ;

作者机构: 1.Zhejiang Acad Agr Sci, Inst Crop & Nucl Technol Utilizat, Hangzhou 310021, Peoples R China

2.Zhejiang Acad Agr Sci, Key Lab Traceabil Agr Genet Modified Organisms, Minist Agr & Rural Affairs, Hangzhou 310021, Peoples R China

3.Zhejiang Acad Agr Sci, State Key Lab Qual & Safety Agroprod, Hangzhou 310021, Peoples R China

关键词: rice quality; Wx; SSIIa; GS3; GW7; allelic combination

期刊名称:INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES ( 影响因子:4.9; 五年影响因子:5.7 )

ISSN: 1661-6596

年卷期: 2025 年 26 卷 14 期

页码:

收录情况: SCI

摘要: As living standards rise, there has been a growing emphasis on quality traits related to rice's taste potential. Recent studies explored correlations among quality traits, but the influence of major genes governing a specific quality trait on other quality traits remains elusive. Here, we report on the application of grain quality genes, two of Waxy (Wx) and Starch synthase II-a (SSIIa), which dominates in rice cooking and eating quality, and two grain length/width-related genes, grain size 3 (GS3) and grain width 7 (GW7), on appearance quality traits. Five allele-specific markers for these genes were developed, and used to stack the desirable alleles at these loci. The effects of individual or combined alleles at the loci were evaluated using a set of 156 rice germplasm. We found that the Wx-In1 (Intron 1) locus exerts a major effect in controlling both amylose content and gel consistency, while the SSIIa-Ex8 (Exon 8) locus primarily governs alkali spreading value. The impact on chalkiness-related traits follows the hierarchy of Wx-In1 > SSIIa-Ex8 > Wx-Ex10 (Exon 10). GS3-Ex2 (Exon 2) has a highly significant impact on chalkiness-related traits, and the GW7-Pro (Promoter) locus exerts a synergistic effect. The GS3-Ex2 locus exerts an effect in controlling both gel consistency and alkali spreading value, while the GW7-Pro locus governs amylose content. The data for newly developed allele-specific markers will facilitate the improvement of rice quality in rice.

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