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Polymorphisms in the 5 ' flanking region of the insulin-like growth factor I gene are associated with growth traits in largemouth bass Micropterus salmoides

文献类型： 外文期刊

作者： Li, Xiao Hui 1 ; Bai, Jun Jie 1 ; Ye, Xing 1 ; Hu, Yin Chang 1 ; Li, Sheng Jie 1 ; Yu, Ling Yun 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Key Lab Trop & Subtrop Fish Breeding & Cultivat, CAFS, Pearl River Fisheries Res Inst, Guangzhou 510380, Guangdong, Peoples R China

关键词： haplotype;growth trait

期刊名称：FISHERIES SCIENCE （ 影响因子：1.617； 五年影响因子：1.531 ）

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收录情况： SCI

摘要： Insulin-like growth factor I (IGF-1) plays an important role in fish growth and development. As the promoter of IGF-I regulates the expression of IGF-I, polymorphisms in the promoter region could be functionally important to differences in IGF-I expression and function. In this study, the 5' flanking region (1,770 bp) of the largemouth bass IGF-I gene was cloned, sequenced and characterized. Several putative liver-enriched transcriptional factor binding sites, including HNF-1 alpha, HNF-3 beta and C/EBP, were found to have multiple copies in the promoter sequence of the largemouth bass IGF-I gene. It did not contain consensus TATA, CCAAT-like or GAGA sequences. Sequencing of 5' flanking sequences from individual fish revealed the presence of a deletion (GTTT) and two single-nucleotide substitutions, causing two different haplotypes: ATTTTTGTTTTT(A) and ATAATT-TT(B). The haplotypes were tested for their association with growth traits in a population of 91 largemouth bass individuals using polymerase chain reaction. The association analysis was carried out using a general linear model. Results showed the haplotypes had significant effects on body weight and body width (P < 0.05). The individuals with the AA genotype had significantly higher body weight and body width than those with the AB and BB genotypes (P < 0.05). Moreover, the effect of the genotypes on IGF-I mRNA expression in the liver was evaluated through the use of a semiquantitative RT-PCR method. The results showed that the IGF-I mRNA expression levels were affected by the promoter polymorphisms (P < 0.01), and that levels of IGF-I mRNA of the AA genotype in liver were greater than those of both the AB and the BB genotypes (P < 0.05 and P < 0.01). Our results implied that the IGF-I gene could be a potential quantitative trait nucleotide that affects growth traits in largemouth bass.

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