文献类型： 外文期刊

作者： Ye, L. Z. ² ; Niu, B. Y. ² ; Luo, L. F. ² ; Shao, G. C. ² ; Mei, S. Q. ¹ ; Deng, C. Y. ² ; Jiang, S. W. ² ; Xiong, Y. Z.; ¹ ;

作者机构： 1.Huazhong Agr Univ, Coll Anim Sci, Key Lab Pig Genet & Breeding, Minist Agr, Wuhan 430070, Peoples R China

2.Huazhong Agr Univ, Key Lab Agr Anim Genet Breeding & Reprod, Minist Educ, Wuhan 430070, Peoples R China

3.NE Agr Univ, Coll Anim Sci & Technol, Harbin 150030, Peoples R China

4.Hubei A

关键词： prei3;ESR;Genotyping;Multiplex PCR;Reproductive traits

期刊名称：LIVESTOCK SCIENCE （ 影响因子：1.943； 五年影响因子：2.252 ）

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收录情况： SCI

摘要： A method allowing simultaneous genotyping of two loci: pre-implantation protein 3 (prei3) and estrogen receptor (ESR) was presented. In multiplex PCR amplification, two amplicons were simultaneously produced a 294 bp fragment of prei3 gene and a 121 bp fragment of ESR gene and were then Subjected to "one-tube" restriction enzyme digestion with Mspl and Pvull. A total of 92 sows from the 4th dam line of Chinese lean-type new lines (DIV line) and 98 gilts from Large White x Meishan (LW x M) F-2 population were genotyped to demonstrate reliability, convenience and lower costs. Statistical analysis showed that no significant difference had been detected among three genotypes at individual locus; but, for second and subsequent litters in both the DIV line, total number of piglets born (TNB) and the number of piglets born alive (NBA) of TTBB and GGAB sows were significantly larger than those of TGBB animals (p<0.05); and in LW x M F-2 offspring, the uterine weight (UW) of TGAA gilts were heavier than those of TTAA gilts (p<0.1), the length of oviduct (LO) of GGAB gilts were longer than those of GGBB gilts, and the weight of both ovaries (OW) of TGAB and GGAA gilts were significantly heavier than those of TGBB gilts (p<0.1). Results here suggested that this method might be useful in the wide-scale genotyping of both loci in pig breeding programs.