Transcriptome analysis of Lr19-virulent mutants provides clues for the AvrLr19 of Puccinia triticina
文献类型： 外文期刊
作者： Cui, Zhongchi 1 ; Wu, Wenyue 1 ; Fan, Fan 1 ; Wang, Fei 1 ; Liu, Daqun 1 ; Di, Dianping 2 ; Wang, Haiyan 1 ;
作者机构： 1.Hebei Agr Univ, Coll Plant Protect, Technol Innovat Ctr Biol Control Crop Dis & Insect, Baoding, Hebei, Peoples R China
2.Hebei Acad Agr & Forestry Sci, Plant Protect Inst, Baoding, Hebei, Peoples R China
关键词： wheat; Puccinia triticina; single nucleotide polymorphism; sequence insertion or deletion; AvrLr19 candidates
期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.2； 五年影响因子：6.2 ）
ISSN：
年卷期： 2023 年 14 卷
页码：
收录情况： SCI
摘要： IntroductionWheat leaf rust caused by Puccinia triticina (Pt) remains one of the most destructive diseases of common wheat worldwide. Understanding the pathogenicity mechanisms of Pt is important to control wheat leaf rust. MethodsThe urediniospores of Pt race PHNT (wheat leaf rust resistance gene Lr19-avirulent isolate) were mutagenized with ethyl methanesulfonate (EMS), and two Lr19-virulent mutants named M1 and M2 were isolated. RNA sequencing was performed on samples collected from wheat cultivars Chinese Spring and TcLr19 infected with wild-type (WT) PHNT, M1, and M2 isolates at 14 days post-inoculation (dpi), respectively. Screening AvrLr19 candidates by quantitative reverse transcription PCR (qPCR) and Agrobacterium-mediated transient assays in Nicotiana benthamiana. Results560 genes with single nucleotide polymorphisms (SNPs) and insertions or deletions (Indels) from non-differentially expressed genes were identified. Among them, 10 secreted proteins were screened based on their fragments per kilobase of exon model per million mapped reads (FPKM) values in the database. qPCR results showed that the expression profiles of 7 secreted proteins including PTTG_27471, PTTG_12441, PTTG_28324, PTTG_26499, PTTG_06910, PTTG_26516, and PTTG_03570 among 10 secreted proteins in mutants were significantly different with that in wild-type isolate after infection wheat TcLr19 and might be related to the recognition between Lr19 and AvrLr19. In addition, a total of 216 differentially expressed genes (DEGs) were obtained from three different sample comparisons including M1-vs-WT, M2-vs-WT, and M1-vs-M2. Among 216 DEGs, 15 were predicted to be secreted proteins. One secreted protein named PTTG_04779 could inhibit programmed progress of cell death (PCD) induced by apoptosis-controlling genes B-cell lymphoma-2 associated X protein (BAX) on Nicotiana benthamiana, indicating that it might play a virulence function in plant. Taken together, total 8 secreted proteins, PTTG_04779, PTTG_27471, PTTG_12441, PTTG_28324, PTTG_26499, PTTG_06910, PTTG_26516, PTTG_03570 are identified as AvrLr19 candidates. DiscussionOur results showed that a large number of genes participate in the interaction between Pt and TcLr19, which will provide valuable resources for the identification of AvrLr19 candidates and pathogenesis-related genes.
- 相关文献
作者其他论文 更多>>
-
Maize lipid droplet-associated protein 2 is recruited by a virus to enhance viral multiplication and infection through regulating cellular fatty acid metabolism
作者：Wang, Siyuan;Wang, Xinyu;Li, Siqi;Sun, Xi;Xue, Mingshuo;Zhou, Tao;Fan, Zaifeng;Wang, Siyuan;Wang, Xinyu;Li, Siqi;Sun, Xi;Xue, Mingshuo;Zhou, Tao;Fan, Zaifeng;Di, Dianping;Zhang, Aihong;Zhang, Yongjiang;Xia, Yiji;Xia, Yiji;Fan, Zaifeng
关键词：rice black streaked dwarf virus (RBSDV); C18 polyunsaturated fatty acids; lipid droplets (LDs); LD-associated protein; Zea mays
-
Genomic prediction of yield performance among single-cross maize hybrids using a partial diallel cross design
作者：Luo, Ping;Wang, Houwen;Yang, Ruisi;Wang, Fei;Yong, Hongjun;Zhou, Zhiqiang;Li, Mingshun;Zhang, Degui;Han, Jienan;Chen, Yong;Zhang, Runze;Gao, Wenwei;Chen, Xiaoyu;Li, Wenjie;Hao, Zhuanfang;Fu, Junjie;Li, Xinhai;Luo, Ping;Zhang, Xuecai;Ni, Zhiyong;Zhang, Lin;Song, Wei;Wang, Liwei;Yang, Jie;Weng, Jianfeng;Meng, Zhaodong;Zhao, Meng
关键词：Maize; Genomic prediction; Prediction model; Genetic effects; Hybrid performance
-
Wheat leaf rust fungus effector Pt13024 is avirulent to TcLr30
作者：Qi, Yue;Li, Jianyuan;Mapuranga, Johannes;Zhang, Na;Chang, Jiaying;Zhang, Yue;Wei, Jie;Cui, Liping;Liu, Daqun;Yang, Wenxiang;Qi, Yue;Li, Jianyuan;Shen, Qianhua;Zhang, Yue;Wei, Jie
关键词：Puccinia triticina; effector; wheat rust; host-pathogen interaction; biotroph; pathogenicity
-
De novo genome assembly of Bradysia cellarum (Diptera: Sciaridae), a notorious pest in traditional special vegetables in China
作者：Li, Mengyao;Fan, Fan;Ge, Yafei;Wei, Guoshu;Yang, Xiaofan;Hong, Dawei;Wang, Zhongyan;Lu, Chenyan;Chen, Suyi
关键词：Bradysia cellarum (odoriphaga); functional annotation; genome assembly; insecticide resistance; PacBio sequencing
-
TaPR1 Interacts With TaTLP1 via the aIV Helix to Be Involved in Wheat Defense to Puccinia triticina Through the CAPE1 Motif
作者：Wang, Fei;Shen, Songsong;Zhao, Cunpeng;Cui, Zhongchi;Meng, Linshuo;Wu, Wenyue;Liu, Daqun;Wang, Haiyan;Zhao, Cunpeng
关键词：TaPR1-4; alpha IV helix; CAPE1; resistance mechanisms; Triticum aestivum; Puccinia triticina
-
Comparative Proteomic Analyses of Susceptible and Resistant Maize Inbred Lines at the Stage of Enations Forming following Infection by Rice Black-Streaked Dwarf Virus
作者：Wang, Rong;Wang, Rong;Du, Kaitong;Jiang, Tong;Fan, Zaifeng;Zhou, Tao;Wang, Rong;Du, Kaitong;Jiang, Tong;Fan, Zaifeng;Zhou, Tao;Di, Dianping
关键词：rice black-streaked dwarf virus; Zea mays; comparative proteome; enation structure; differentially expressed protein; stress
-
Evaluation of the resistance to Chinese predominant races of Puccinia triticina and analysis of effective leaf rust resistance genes in wheat accessions from the US National Plant Germplasm System
作者：Zhang, Lin;Zhao, Xuefang;Yan, Hongfei;Meng, Qingfang;Liu, Daqun;Zhang, Lin;Liu, Jingxian;Wang, Xiaolu;Gong, Wenping;Wang, Xiaolu;Gong, Wenping;Wang, Xiaolu;Gong, Wenping;Zhang, Quanguo;Liu, Yuping
关键词：leaf rust; wheat accessions; resistance gene; molecular markers; races
