您好，欢迎访问江苏省农业科学院 机构知识库！
江苏省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

NRAS promotes the proliferation of melanocytes to increase melanin deposition in Rex rabbits

文献类型： 外文期刊

作者： Bai, Shaocheng 1 ; Hu, Shuaishuai 1 ; Dai, Yingying 1 ; Jin, Rongshuai 1 ; Zhang, Chen 1 ; Yao, Fan 1 ; Weng, Qiaoqin 2 ; Zhai, Pin 3 ; Zhao, Bohao 1 ; Wu, Xinsheng 1 ; Chen, Yang 1 ;

作者机构： 1.Yangzhou Univ, Coll Anim Sci & Technol, Yangzhou 225000, Peoples R China

2.Yuyao Xinnong Rabbit Ind Co Ltd, Ningbo 315400, Peoples R China

3.Jiangsu Acad Agr Sci, Nanjing 210000, Peoples R China

4.Yangzhou Univ, Joint Int Res Lab Agr & Agriprod Safety, Yangzhou 225000, Peoples R China

关键词： NRAS; melanocytes; melanin; Rex rabbit; melanin-related genes

期刊名称：GENOME （ 影响因子：2.449； 五年影响因子：2.665 ）

ISSN： 0831-2796

年卷期：

页码：

收录情况： SCI

摘要： Melanocytes play a major role in the formation of mammalian fur color and are regulated by several genes. Despite playing the pivotal role in the study of melanoma, the mechanistic role of NRAS (neuroblastoma RAS viral oncogene homolog) in the formation of mammalian epidermal color is still elusive. First of all, the expression levels of NRAS mRNA and protein in the dorsal skin of different colored Rex rabbits were detected by qRT-PCR and Western blot. Then, the subcellular localization of NRAS was identified in melanocytes by indirect immunofluorescence. Next, the expression of NRAS was overexpressed and knocked down in melanocytes, and its efficiency was verified by qRT-PCR and Western blot. Subsequently, NaOH, CCK-8, and Annexin V-FITC were used to verify the changes in melanin content, proliferation, and apoptosis in melanocytes. Finally, we analyzed the regulation of NRAS on other genes (MITF, TYR, DCT, PMEL, and CREB) that affect melanin production. In silico studies showed NRAS as a stable and hydrophilic protein, and it is localized in the cytoplasm and nucleus of melanocytes. The mRNA and protein expression levels of NRAS were significantly different in skin of different colored Rex rabbits, and the highest level was found in black skin (P < 0.01). Moreover, the NRAS demonstrated impact on the proliferation, apoptosis, and melanin production of melanocytes (P < 0.05), and the strong correlation of NRAS with melanin-related genes was evidently observed (P < 0.05). Our results suggested that NRAS can be used as a gene that regulates melanin production and controls melanocyte proliferation and apoptosis, providing a new theoretical basis for studying the mechanism of mammalian fur color formation.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江苏省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台