Phylogenetic Variation of Tri1 Gene and Development of PCR-RFLP Analysis for the Identification of NX Genotypes in Fusarium graminearum Species Complex
文献类型： 外文期刊
作者： Gao, Meiling 1 ; Zhang, Mengyuan 1 ; Zhang, Jiahui 1 ; Yang, Xianli 1 ; Abdallah, Mohamed F. 3 ; Wang, Jianhua 1 ;
作者机构： 1.Shanghai Acad Agr Sci, Inst Agrofood Stand & Testing Technol, Minist Agr, 1000 Jinqi Rd, Shanghai 201403, Peoples R China
2.Dalian Polytech Univ, Natl Engn Res Ctr Seafood, Sch Food Sci & Technol, Dalian 116034, Peoples R China
3.Univ Ghent, Fac Biosci Engn, Dept Food Technol Safety & Hlth, Coupure Links 653, B-9000 Ghent, Belgium
4.Assiut Univ, Fac Vet Med, Dept Forens Med & Toxicol, Assiut 71515, Egypt
关键词： Fusarium graminearum species complex; trichothecenes; PCR-RFLP; NX toxins
期刊名称：TOXINS （ 影响因子：4.2； 五年影响因子：4.7 ）
ISSN：
年卷期： 2023 年 15 卷 12 期
页码：
收录情况： SCI
摘要： NX toxins have been described as a novel group of type A trichothecenes produced by members of the Fusarium graminearum species complex (FGSC). Differences in structure between NX toxins and the common type B trichothecenes arise from functional variation in the trichothecene biosynthetic enzyme Tri1 in the FGSC. The identified highly conserved changes in the Tri1 gene can be used to develop specific PCR-based assays to identify the NX-producing strains. In this study, the sequences of the Tri1 gene from type B trichothecene- and NX-producing strains were analyzed to identify DNA polymorphisms between the two different kinds of trichothecene producers. Four sets of Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) methods were successfully developed to distinguish the common type B trichothecene producers and NX producers within FGSC. These promising diagnostic methods can be used for high-throughput genotype detection of Fusarium strains as a step forward for crop disease management and mycotoxin control in agriculture. Additionally, it was found that the Tri1 gene phylogeny differs from the species phylogeny, which is consistent with the previous studies.
- 相关文献
作者其他论文 更多>>
-
2D/2D Co3O4/BiOCl nanocomposite with enhanced antibacterial activity under full spectrum: Synergism of mesoporous structure, photothermal effect and photocatalytic reactive oxygen species
作者：Wang, Xuesheng;Zhang, Zhijie;Shao, Yi;Yao, Chunxia;Huang, Liujuan;Song, Wei;Yang, Xianli
关键词：BiOCl; Photothermal effect; Antibacterial; Photocatalysis
-
Occurrence of Co-Contamination and Interaction of Multi-Mycotoxins in Dairy Cow Feed in China
作者：Zhu, Zuoyin;Cheng, Haisheng;Wang, Jie;Zhou, Xinli;Zhu, Zuoyin;Cheng, Haisheng;Wang, Jie;Wang, Jianhua;Yang, Junhua;Ma, Junmei;Wang, Hongyang
关键词：feed; mycotoxin; co-contamination; correlation; interaction
-
Inhibitory Effect of Volatiles from Flavobacterium johnsoniae TR-18 on Aspergillus flavus Growth and Aflatoxin Production
作者：Gong, Andong;Liu, Jingrong;Wang, Gaozhan;Song, Mengge;Wang, Jianhua;Zhang, Jingbo
关键词：aflatoxins;
Aspergillus flavus ;Flavobacterium johnsoniae ; inhibition; volatiles -
Integrated Cytotoxicity and Metabolomics Analysis Reveals Cell-Type-Specific Responses to Co-Exposure of T-2 and HT-2 Toxins
作者：He, Weihua;Wang, Qinggong;Zhai, Xiaohu;Zhu, Zuoyin;Xu, Jingru;Huang, Chengbao;Wang, Jianhua;Yang, Junhua
关键词：T-2 toxin; HT-2 toxin; porcine cells; metabolic pathways; biomarkers; combined toxicity
-
RNAi technologies for insect control in crop protection
作者：Zhong, Xue;Xu, Jiajing;Qin, Mengchao;Cao, Mingxin;Xia, Lanqin;Zhong, Xue;Francis, Frederic;Zhong, Xue;Xu, Jiajing;Qin, Mengchao;Cao, Mingxin;Xia, Lanqin;Yu, Xiudao;Zhang, Jiahui
关键词：Crop protection; Double-stranded RNA (dsRNA); Insect control; RNA interference (RNAi)
-
Assessing safety risks associated with Burkholderia gladiolus in soaked Auricularia auricula-judae: One instant predictive model study
作者：Chen, Xiujin;Dai, Minghui;Dai, Minghui;Qu, Yang;Chen, Shanshan;Yang, Xianli;Bai, Yalong;Zhou, Changyan;Suo, Yujuan;Bai, Li;Peng, Zixin;Wang, Jun
关键词：Auricularia auricula-judae; Burkholderia gladioli; Predictive model; Bongkrekic acid; Foodborne pathogen
-
Population Genetic Analyses and Trichothecene Genotype Profiling of Fusarium pseudograminearum Causing Wheat Crown Rot in Henan, China
作者：Zhang, Jianzhou;Li, Chunying;Zhang, Jiahui;Song, Fengping;Wang, Jianhua;Zhang, Mengyuan;Wang, Wenyu;Suo, Yujuan
关键词：Fusarium crown rot; Fusarium pseudograminearum; trichothecene genotype; mating type
