Multi-omics-based identification of purple acid phosphatases and metabolites involved in phosphorus recycling in stylo root exudates
文献类型： 外文期刊
作者： Wu, Yuanhang 1 ; Zhao, Cang 1 ; Zhao, Xingkun 1 ; Yang, Liyun 1 ; Liu, Chun 1 ; Jiang, Lingyan 1 ; Liu, Guodao 1 ; Liu, Pandao 3 ; Luo, Lijuan 1 ;
作者机构： 1.Hainan Univ, Coll Trop Crops, Haikou 570228, Peoples R China
2.Chinese Acad Trop Agr Sci, Trop Crops Genet Resources Inst, Haikou 571101, Peoples R China
3.Hainan Univ, Sanya Nanfan Res Inst, Sanya 572025, Peoples R China
4.58 Renmin Ave, Haikou, Hainan, Peoples R China
5.4 West Xueyuan Rd, Haikou, Hainan, Peoples R China
关键词： Purple acid phosphatase; Root exudates; Low phosphate stress
期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.2； 五年影响因子：7.8 ）
ISSN： 0141-8130
年卷期： 2023 年 241 卷
页码：
收录情况： SCI
摘要： Stylo (Stylosanthes guianensis) is a tropical forage and cover crop that possesses low phosphate (Pi) tolerance traits. However, the mechanisms underlying its tolerance to low-Pi stress, particularly the role of root exudates, remain unclear. This study employed an integrated approach using physiological, biochemical, multi-omics, and gene function analyses to investigate the role of stylo root exudates in response to low-Pi stress. Widely targeted metabolomic analysis revealed that eight organic acids and one amino acid (L-cysteine) were significantly increased in the root exudates of Pi-deficient seedlings, among which tartaric acid and L-cysteine had strong abilities to dissolve insoluble-P. Furthermore, flavonoid-targeted metabolomic analysis identified 18 flavonoids that were significantly increased in root exudates under low-Pi conditions, mainly belonging to the isoflavonoid and flavanone subclasses. Additionally, transcriptomic analysis revealed that 15 genes encoding purple acid phosphatases (PAPs) had upregulated expression in roots under low-Pi conditions. Among them, SgPAP10 was characterized as a root-secreted phosphatase, and overexpression of SgPAP10 enhanced organic-P utilization by transgenic Arabidopsis. Overall, these findings provide detailed information regarding the importance of stylo root exudates in adaptation to low-Pi stress, highlighting the plant's ability to release Pi from organic-P and insoluble-P sources through root-secreted organic acids, amino acids, flavonoids, and PAPs.
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