Physiological characteristics and transcriptomic analyses of alfalfa root crown in wintering
文献类型： 外文期刊
作者： Wang, Xiaolong 1 ; Chai, Hua 1 ; Li, Shasha 1 ; Xu, Yanxia 1 ; Wu, Yue 1 ; Wang, Jianli 2 ; Yang, Zhao 1 ;
作者机构： 1.Heilongjiang Acad Agr Sci, Branch Anim Husb & Vet, Qiqihar, Heilongjiang, Peoples R China
2.Heilongjiang Acad Agr Sci, Inst Grass Sci, Harbin, Heilongjiang, Peoples R China
关键词： alfalfa; cold resistance; physiological characteristics; RNA sequencing (RNA-seq); differentially expressed genes (DEGs); winter survival rate
期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：4.8； 五年影响因子：5.7 ）
ISSN： 1664-462X
年卷期： 2024 年 15 卷
页码：
收录情况： SCI
摘要： Background Alfalfa, scientifically identified as Medicago sativa, is repeatedly referred to as the "king of forages". Because of its tight relationship to winter hardiness, the alfalfa's root crown plays a significant role as a storage organ over the winter. At present, it is still unknown what molecular process makes the alfalfa root crown resistant to cold. This study was aimed to study these knowledge gaps. Using RNA sequencing (RNA-Seq) technology, significant genes associated with cold hardiness were found.Methods According to the random block design, Longmu 806 alfalfa and Sardi alfalfa were planted in regional experiments. Under the condition of low-temperature treatment in winter, the differentially expressed genes (DEGs), winter survival rate (WSR), and physiological characteristics were, in turn, calculated by RNA-Seq, chemical analysis, and field investigation.Results The WSR of the Longmu 806 alfalfa was 3.68-fold greater than that of the Sardi alfalfa. The jasmonic acid (JA), soluble sugar (SS), proline (Pro), and glutathione (GSH) concentration in the roots of Longmu 806 alfalfa was more than the same amount in Sardi alfalfa in other words P is less than 0.05. An entire set of 878 DEGs related to winter hardiness was found by statistical analysis. Among them, 463 DEGs showed an increase in expression, whereas 415 DEGs showed a decrease in expression. The metabolic pathways' examination presented that the DEGs (MsERF1, MsCHIB, MsJAZ, MsAOC, MsGST, MsINV, MsTPS, and MsOAT) were linked to the pathways of "plant hormone signaling transduction", "Amino sugar and nucleotide sugar metabolism", and "glutathione metabolism". Furthermore, the physiological changes in JA, SS, Pro content, and GSH were influenced by the dynamic transcription profile of LT (low- temperature) resistance-related genes.
- 相关文献
作者其他论文 更多>>
-
The synergistic roles of MsRCI2B and MsRCI2E in the regulation of ion balance and ROS homeostasis in alfalfa under salt stress
作者：Zhang, Depeng;He, Pin;Li, Donghuan;Meng, Jing;Luo, Yaqin;Wang, Xinsheng;Zhang, Shichao;Cai, Hua;Shen, Zhongbao;Wang, Jianli;Zhang, Dongmei;You, Jia;Zhuang, Xu;Mu, Linlin;Han, Weibo
关键词：Medicago sativa; RCI2; Salt stress
-
Nonlinear responses of ecosystem carbon fluxes to precipitation change in a semiarid grassland
作者：Chai, Hua;Li, Jie;Zhou, Xuhui;Chai, Hua;Li, Junqin;Meng, Bo;Wang, Chengliang;Sun, Wei;Ma, Jianying;Zhang, Jinwei;Pan, Duofeng
关键词：ecosystem carbon fluxes; altered precipitation; nonlinear responses; precipitation gradient; semiarid grasslands
-
Transcriptomic Analysis Identifies Molecular Response of the Tolerant Alfalfa (Medicago sativa) Cultivar Nongjing 1 to Saline-Alkali Stress
作者：Zhang, Dongmei;Shen, Zhongbao;Han, Weibo;Mou, Linlin;Zhuang, Xu;Wang, Jianli;Li, Jinxia;Zhang, Yiming;Zhang, Yuanhao;Wang, Wenhui;Li, Zhaohui;Zhu, Peng;Huang, Yongshun;Han, Long;Wang, Mingyu;Zhang, Zijian;Pang, Qiuying;Li, Lixin;Li, Jinxia
关键词：
Medicago sativa ; saline-alkali adaptation; yield; flavonoid biosynthesis; transcriptional regulation -
Comparative transcriptome analysis of differentially expressed genes of Medicago falcata L. breeding lines response to saline-alkaline stress
作者：Chai, Hua;Wang, Xiaolong;Yang, Zhao;Li, Shasha;Xu, Yanxia;Wu, Yue;Chai, Hua;Shen, ZhongBao
关键词：
Medicago falcata L.; Salt-alkali stress; RNA-seq; Transcription factor; WGCNA -
The synergistic roles of MsRCI2B and MsRCI2E in the regulation of ion balance and ROS homeostasis in alfalfa under salt stress (vol 300, 140093, 2025)
作者：Zhang, Depeng;He, Pin;Li, Donghuan;Meng, Jing;Luo, Yaqin;Wang, Xinsheng;Zhang, Shichao;Cai, Hua;Shen, Zhongbao;Wang, Jianli;Zhang, Dongmei;You, Jia;Zhuang, Xu;Mu, Linlin;Han, Weibo
关键词：
-
Analysis of physiological characteristics and gene co-expression networks in Medicago sativa roots under low-temperature stress
作者：Wang, Xiaolong;Chai, Hua;Xu, Yanxia;Li, Shasha;Wu, Yue;Wang, Ruoding;Yang, Zhao
关键词：
Medicago sativa ; physiological characteristics; genes; cold tolerance; correlation analysis -
Developing a highly efficient transgenic approach to generate sheepgrass germplasm with enhanced biomass yield and salt tolerance
作者：Zhao, Haixia;Jiang, Xiping;Yang, Ruijuan;Wu, Zhenying;Fu, Chunxiang;Zhao, Haixia;Jiang, Xiping;Yang, Ruijuan;Wu, Zhenying;Fu, Chunxiang;Zhao, Haixia;Wu, Zhenying;Fu, Chunxiang;You, Jia;Wang, Jianli;Shen, Zhongbao;Liu, Yaling;Yuan, Feng;Wang, Yi;Zheng, Wendong
关键词：
