A chromosome-level reference genome facilitates the discovery of clubroot-resistant gene Crr5 in Chinese cabbage
文献类型： 外文期刊
作者： Yang, Shuangjuan 1 ; Wang, Xiangfeng 2 ; Wang, Zhaojun 3 ; Zhang, Wenjing 1 ; Su, Henan 1 ; Wei, Xiaochun 1 ; Zhao, Yanyan 1 ; Wang, Zhiyong 1 ; Zhang, Xiaowei 1 ; Guo, Li 2 ; Yuan, Yuxiang 1 ;
作者机构： 1.Henan Acad Agr Sci, Inst Vegetables, 116 Huanyuan Rd, Zhengzhou 450002, Henan, Peoples R China
2.Peking Univ, Inst Adv Agr Sci, Shandong Key Lab Precis Mol Crop Design & Breeding, Shandong Lab Adv Agr Sci Weifang, 699 Binhu Rd, Weifang 261325, Shandong, Peoples R China
3.Henan Agr Univ, Coll Tobacco Sci, 218 Pingan Rd, Zhengzhou 450046, Henan, Peoples R China
期刊名称：HORTICULTURE RESEARCH （ 影响因子：8.5； 五年影响因子：9.1 ）
ISSN： 2662-6810
年卷期： 2025 年 12 卷 3 期
页码：
收录情况： SCI
摘要： Brassica rapa includes a variety of important vegetable and oilseed crops, yet it is significantly challenged by clubroot disease. Notably, the majority of genotypes of B. rapa with published genomes exhibit high susceptibility to clubroot disease. The present study presents a high-quality chromosome-level sequence of the genome of the DH40 clubroot-resistant (CR) line, a doubled haploid line derived from the hybrid progeny of a European turnip (ECD01) and two lines of Chinese cabbage. The assembled genome spans 420.92 Mb, with a contig N50 size of 11.97 Mb. Comparative genomics studies revealed that the DH40 line is more closely related to the Chinese cabbage Chiifu than to the turnip ECD04. The DH40 genome provided direct reference and greatly facilitate the map-based cloning of the clubroot resistance gene Crr5, encoding a nucleotide-binding leucine-rich repeat (NLR) protein. Further functional analysis demonstrated that Crr5 confers clubroot resistance in both Chinese cabbage and transgenic Arabidopsis. It responds to inoculation with Plasmodiophora brassicae and is expressed in both roots and leaves. Subcellular localization shows that Crr5 is present in the nucleus. Notably, the Toll/interleukin-1 receptor (TIR) domain of Crr5 can autoactivate and trigger cell death. In addition, we developed two Crr5-specific Kompetitive allele-specific PCR (KASP) markers and showcased their successful application in breeding CR Chinese cabbage through marker-assisted selection. Overall, our research offers valuable resources for genetic and genomic studies in B. rapa and deepens our understanding of the molecular mechanisms underlying clubroot resistance against P. brassicae.
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