Overexpression of CYP6CX4 contributing to field-evolved resistance to flupyradifurone, one novel butenolide insecticide, in Bemisia tabaci from China
文献类型： 外文期刊
作者： Zhang, Qinghe 1 ; Tang, Juan 1 ; Gao, Bingli 1 ; Qu, Cheng 1 ; Wang, Jinda 3 ; Luo, Chen 1 ; Wang, Ran 1 ;
作者机构： 1.Beijing Acad Agr & Forestry Sci, Inst Plant Protect, Beijing 100097, Peoples R China
2.Yangtze Univ, Coll Agr, Jingzhou 434025, Peoples R China
3.Fujian Agr & Forestry Univ, Natl Engn Res Ctr Sugarcane, Fuzhou 350002, Peoples R China
关键词： Bemisia tabaci; Flupyradifurone; Cytochrome P450
期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.2； 五年影响因子：7.8 ）
ISSN： 0141-8130
年卷期： 2024 年 265 卷
页码：
收录情况： SCI
摘要： Bemisia tabaci is a formidable insect pest worldwide, and exhibits significant resistance to various insecticides. Flupyradifurone is one novel butenolide insecticide and has emerged as a new weapon against B. tabaci , but fieldevolved resistance to this insecticide has become a widespread concern. To unravel the mechanisms of fieldevolved flupyradifurone resistance, we conducted a comprehensive investigation into susceptibility of twentyone field populations within the Beijing -Tianjin -Hebei Region of China. Alarmingly, thirteen of these populations displayed varying degrees of resistance, ranging from low to medium levels, and building upon our prior findings, we meticulously cloned and characterized the CYP6CX4 gene in B. tabaci . Our investigations unequivocally confirmed the association between CYP6CX4 overexpression and flupyradifurone resistance in three of the thirteen resistant strains via RNA interference. To further validate our findings, we introduced CYP6CX4 overexpression into a transgenic Drosophila melanogaster line, resulting in a significant development of resistance to flupyradifurone in D. melanogaster . Additionally, homology modeling and molecular docking analyses showed the stable binding of flupyradifurone to CYP6CX4 , with binding free energy of -6.72 kcal mol - 1 . Collectively, our findings indicate that the induction of CYP6CX4 exerts one important role in detoxification of flupyradifurone, thereby promoting development of resistance in B. tabaci.
- 相关文献
作者其他论文 更多>>
-
Circabidian rhythm of sex pheromone reception in a scarab beetle
作者：Wang, Yinliang;Dong, Huanhuan;Qu, Yafei;Qin, Jianhui;Li, Kebin;Cao, Yazhong;Zhang, Shuai;Yin, Jiao;Wang, Yinliang;Dong, Huanhuan;Zhou, Yuxin;Ren, Bingzhong;Luo, Chen;Leal, Walter S.
关键词：
-
Tetraniliprole risk assessment: Unveiling a hidden threat for managing a generalist herbivore
作者：Qu, Cheng;Li, Yunyi;Zhan, Qianyuan;Luo, Chen;Wang, Ran;Qu, Cheng;Wang, Ran;Wang, Jinda;Guedes, Raul Narciso C.
关键词：Tobacco cutworm; Realized heritability; Baseline of susceptibility; Mode of inheritance; Biochemical mechanism; Transgenerational hormesis
-
Risk assessment, cross-resistance, biochemical mechanism and fitness cost of tetraniliprole resistance in the tomato pinworm Tuta absoluta
作者：Qu, Cheng;Yao, Jiaqi;Zhan, Qianyuan;Zhang, Daofeng;Li, Yunyi;Wang, Ran;Qu, Cheng;Yao, Jiaqi;Zhan, Qianyuan;Zhang, Daofeng;Li, Yunyi;Wang, Ran;Yao, Jiaqi;Huang, Jianlei
关键词：Tuta absoluta; Tetraniliprole; Risk assessment; Cross -resistance; Biochemical mechanism; Fitness cost
-
Baseline of susceptibility, risk assessment, biochemical mechanism, and fitness cost of resistance to dimpropyridaz, a novel pyridazine pyrazolecarboxamide insecticide, in Bemisia tabaci from China
作者：Tang, Juan;Qu, Cheng;Zhan, Qianyuan;Zhang, Daofeng;Luo, Chen;Wang, Ran;Tang, Juan;Qu, Cheng;Zhan, Qianyuan;Zhang, Daofeng;Luo, Chen;Wang, Ran;Wang, Jinda
关键词：Bemisia tabaci; Dimpropyridaz; Baseline of susceptibility; Risk assessment; Fitness cost; Detoxifying enzymes
-
Orco mediates olfactory behavior and oviposition in the whitefly Bemisia tabaci
作者：Li, Fengqi;Luo, Chen;Li, Fengqi;Tian, Jiahui;Di, Zhongjuan;Qu, Cheng;Luo, Chen;Fu, Yuejun;Yang, Shiyong
关键词：Whitefly; Orco; Behavior; Host recognition; Oviposition; RNAi
-
Knockdown of one cytochrome P450 gene CYP6DW4 increases the susceptibility of Bemisia tabaci to dimpropyridaz, a novel pyridazine pyrazolecarboxamide insecticide
作者：Tang, Juan;Su, Qi;Tang, Juan;Zhang, Qinghe;Qu, Cheng;Luo, Chen;Wang, Ran;Tang, Juan;Zhang, Qinghe;Qu, Cheng;Luo, Chen;Wang, Ran
关键词：Bemisia tabaci; Dimpropyridaz; Insecticide detoxification; Cytochrome P450 monooxygenase
-
Tetraniliprole resistance in field-collected populations of Tuta absoluta (Lepidoptera: Gelechiidae) from China: Baseline susceptibility, cross-resistance, inheritance, and biochemical mechanism
作者：Qu, Cheng;Yao, Jiaqi;Luo, Chen;Wang, Ran;Yao, Jiaqi;Huang, Jianlei;Che, Wunan;Fang, Yong;Fang, Yong
关键词：Tuta absoluta; Tetraniliprole; Cross -resistance; Synergism; Metabolic enzyme; Mode of inheritance
