Knockdown of one cytochrome P450 gene CYP6DW4 increases the susceptibility of Bemisia tabaci to dimpropyridaz, a novel pyridazine pyrazolecarboxamide insecticide
文献类型： 外文期刊
作者： Tang, Juan 1 ; Zhang, Qinghe 2 ; Qu, Cheng 2 ; Su, Qi 1 ; Luo, Chen 2 ; Wang, Ran 2 ;
作者机构： 1.Yangtze Univ, Coll Agr, Jingzhou 434000, Peoples R China
2.Beijing Acad Agr & Forestry Sci, Inst Plant Protect, Beijing 100097, Peoples R China
3.Minist Agr & Rural Affairs, Key Lab Environm Friendly Management Fruit & Veget, Beijing 100097, Peoples R China
关键词： Bemisia tabaci; Dimpropyridaz; Insecticide detoxification; Cytochrome P450 monooxygenase
期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：4.7； 五年影响因子：4.7 ）
ISSN： 0048-3575
年卷期： 2024 年 201 卷
页码：
收录情况： SCI
摘要： Bemisia tabaci is a formidable insect pest worldwide, and it exhibits significant resistance to various insecticides. Dimpropyridaz is a novel pyridazine pyrazolecarboxamide insecticide used against sucking insect pests, but there is little information regarding its metabolic detoxification in arthropods or cross-resistance with other insecticides. In this study, we found that dimpropyridaz shows no cross-resistance with three other popular insecticides, namely abamectin, cyantraniliprole, and flupyradifurone. After treatment of B. tabaci adults with a high dose of dimpropyridaz, higher cytochrome P450 monooxygenase (P450) activity was detected in the survivors, and the expression of the P450 gene CYP6DW4 was highly induced. Cloning and characterization of the full-length amino acid sequence of CYP6DW4 indicated that it contains conserved domains typical of P450 genes, phylogenetic analysis revealed that it was closely related to a B. tabaci protein, CYP6DW3, known to be involved in detoxification of imidacloprid. Silencing of CYP6DW4 by feeding insects with dsRNA significantly increased the susceptibility of B. tabaci to dimpropyridaz. In addition, homology modeling and molecular docking analyses showed the stable binding of dimpropyridaz to CYP6DW4, with binding free energy of -6.65 kcal/mol. Our findings indicate that CYP6DW4 plays an important role in detoxification of dimpropyridaz and possibly promotes development of resistance in B. tabaci.
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