您好，欢迎访问浙江省农业科学院 机构知识库！
浙江省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

HDL receptor/Scavenger receptor B1-Scarb1 and Scarb1-like mediate the carotenoid-based red coloration in fish

文献类型： 外文期刊

作者： Du, Jinxing 1 ; Chen, Honglin 1 ; Mandal, Biplab K. 1 ; Wang, Jun 1 ; Shi, Zhiyi 1 ; Lu, Guoqing 2 ; Wang, Chenghui 1 ;

作者机构： 1.Shanghai Ocean Univ, Key Lab Genet Resources Freshwater Aquaculture &, Minist Agr & Rural Affair, Shanghai 201306, Peoples R China

2.Univ Nebraska, Dept Biol, Omaha, NE 68135 USA

3.Chinese Acad Fisheries Sci, Pearl River Fisheries Res Inst, Key Lab Trop & Subtrop Fishery Resource Applicat, China Minist Agr, Guangzhou 510380, Peoples R China

4.Zhejiang Acad Agr Sci, Inst Hydrobiol, Hangzhou 310021, Peoples R China

关键词： Red and white coloration; Scarb1; Gch1; CRISPR/Cas9; Carotenoid metabolism

期刊名称：AQUACULTURE （ 影响因子：5.135； 五年影响因子：5.125 ）

ISSN： 0044-8486

年卷期： 2021 年 545 卷

页码：

收录情况： SCI

摘要： Both carotenoids and pteridine pigments can produce colors ranging from red to yellow and participate in mate choice, antioxidant, and immunity processes in animals. Common carp (Cyprinus carpio) variants with red coloration have been selectively bred for more than a thousand years, yet the mechanism that regulates coloration remains poorly understood. Here we provided experimental evidence that the red coloration in common carp varieties was carotenoid-based and was regulated by the carotenoid transport genes Scarb1 and Scarb1-like (high density lipoprotein (HDL) receptor/scavenger receptor B type 1, also known as SR-BI). We found that carotenoids rather than pteridine pigments were specific to whole red (WR) but absent in whole white (WW) Oujiang color common carp (Cyprinus carpio var. color). Scarb1 and its novel paralog gene Scarb1-like were the most significant carotenoid metabolism-related genes differentially expressed between WR and WW, whereas no difference was detected for the Gch1 (GTP cyclohydrolase 1) gene, which acted as a rate limiting enzyme in the pteridine pathway. Disruption of Scarb1, Scarb1-like, and Gch1 using CRISPR/Cas9 technology resulted in regional red skin fading into white color in Scarb1 and Scarb1-like mutated WR (S+/-), leading to a decrease and a complete loss of astaxanthin and lutein in the red and white areas, respectively. However, no skin color variations were observed in Gch1 mutated WR fishes. In addition, disruption of the two Scarb1 genes and Gch1 together in WR caused red color variations similar to those observed in S+/- WR. Finally, carotenoids supplementation recovered the red color but had poorly effects on the white color in S+/- WR. This study revealed a plausible molecular mechanism responsible for red and white body colors and may benefit selective breeding programs of ornamental and cultured fish.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：浙江省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台