RNA sequencing and functional analysis of adult gonadal tissue to identify candidate key genes in Macrobrachium rosenbergii sex development
文献类型： 外文期刊
作者： Ren, Jindong 1 ; Na, Rong 1 ; Chen, Honglin 1 ; Lou, Bao 1 ; Niu, Baolong 1 ;
作者机构： 1.Zhejiang Acad Agr Sci, Inst Aquat Biol, Hangzhou 310021, Zhejiang, Peoples R China
关键词： Macrobrachium rosenbergii; Transcriptome; Sex development; Transcription factors
期刊名称：AQUACULTURE INTERNATIONAL （ 影响因子：2.235； 五年影响因子：2.616 ）
ISSN： 0967-6120
年卷期： 2021 年 29 卷 6 期
页码：
收录情况： SCI
摘要： Macrobrachium rosenbergii is a typical aquatic organism with reversible gonadal development that is regulated by gene expression. The role of transcription factors in gonadal in adult shrimps remains unclear in M. rosenbergii. In this study, we sequenced the transcriptomes of adult shrimp testes, ovaries, and androgenic glands using second-generation sequencing. In total, 24,007 genes were identified and 9,199 differentially expressed genes (DEGs) were identified by pairwise comparison. There were 272 differentially expressed transcription factors (TFs); 107, 152, and 13 differentially expressed TFs were identified in the testes, ovaries, and androgenic glands by three pairwise comparisons, respectively. GO and KEGG analyses of the TFs and DEGs involved in the MAPK signaling and transcriptional regulation pathways and play key roles in the cell cycle of the testes, whereas involved in the thyroid hormone signaling pathway and neuroactive ligand-receptor interaction play important roles in the ovary. We determined the existence of networks comprising important TFs related to sex development in adult M. rosenbergii gonads. The key TFs were Piwi (expressed only in the testes and ovaries) and Argonaute 3 (expressed only in the ovaries), which might be involved in the regulation of testes and ovary development.
- 相关文献
作者其他论文 更多>>
-
Parentage Determination of Chinese Hooksnout Carp (Opsariichthys bidens) Based on Microsatellite DNA Markers
作者：Ge, Jianhui;Xu, Xiaojun;Ren, Jindong;Lou, Bao;Ge, Jianhui;Shen, Xiaoming;Yu, Jin
关键词：
-
Deep2Pep: A deep learning method in multi-label classification of bioactive peptide
作者：Chen, Lihua;Hu, Zhenkang;Rong, Yuzhi;Lou, Bao
关键词：Bioactive Peptide; Long short term memory (LSTM); Bidirectional Encoder Representation from; Transformers (BERT); Attention; Multi -label
-
A male-specific insert of Opsariichthys bidens identified based on genome-wide association analyses and comparative genomics
作者：Xu, Xiaojun;Ge, Jianhui;Guan, Wenzhi;Niu, Baolong;Lou, Bao;Yu, Jiongying;Yi, Shaokui;Weng, Xudong;Zhang, Xinhui;Zhang, Xinhui
关键词：Opsariichthys bidens; Sex; Growth; GWAS; Sex-specific marker
-
Metabonomics and Transcriptomics Analyses Reveal the Development Process of the Auditory System in the Embryonic Development Period of the Small Yellow Croaker under Background Noise
作者：Jiang, Qinghua;Liang, Xiao;Ye, Ting;Zhang, Yu;Lou, Bao
关键词：Larimichthys polyactis; auditory system; embryonic development; background noise; metabonome; transcriptome
-
Transcriptome analysis revealed changes in multiple genes in Larimichthys crocea under starvation stress
作者：Zhang, Tianle;Zhan, Wei;Xie, Qingping;Ye, Ting;Lou, Bao;Liu, Feng;Tao, Shunshun;Chen, Limin;Shao, Guoer;Lou, Bao;Liu, Feng
关键词：Larimichthys crocea; Starvation stress; RNA-seq; DEGs
-
A chromosome-level genome assembly of East Asia endemic minnow Zacco platypus
作者：Xu, Xiaojun;Guan, Wenzhi;Niu, Baolong;Lou, Bao;Chen, Jing;Yi, Shaokui
关键词：
-
Characterization of the effects of Chinese peony (Radix paeoniae Alba) and its active ingredients on the hematopoietic function of the Chinese mitten crab (Eriocheir sinensis)
作者：Lyu, Sunjian;Shen, Weifeng;Guo, Qi;Han, Mingming;Xiong, Fulei;Liu, Li;Lou, Bao;Bu, Weishao;Yuan, Julin
关键词：Eriocheir sinensis; Hematopoietic tissue; Radix paeoniae Alba; Monomer
