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An engineered Bacillus thuringiensis strain with insecticidal activity against Scarabaeidae (Anomala corpulenta) and Chrysomelidae (Leptinotarsa decemlineata and Colaphellus bowringi)

文献类型： 外文期刊

作者： Yan, Ginxin 1 ; Song, Fuping 1 ; Shu, Changlong 1 ; Liu, Jingjing 1 ; Liu, Chunqin 2 ; Huang, Dafang 3 ; Feng, Shulian 1 ;

作者机构： 1.CAAS, State Key Lab Biol Plant Dis & Insect Pests, Inst Plant Protect, Beijing 100193, Peoples R China

2.Cangzhou Acad Agr & Forestry Sci, Cangzhou 061001, Peoples R China

3.CAAS, Biotechnol Res Inst, Beijing 100081, Peoples R China

4.Hebei Acad Agr & Forestry Sci, Inst Plant Protect, Baoding 071000, Peoples R China

期刊名称：BIOTECHNOLOGY LETTERS （ 影响因子：2.461； 五年影响因子：2.457 ）

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收录情况： SCI

摘要： A genetically-engineered Bacillus thuringiensis (Bt) strain, 3A-HBF, with a broad insecticidal spectrum was constructed by introducing the recombinant plasmid pSTK-3A containing cry3Aa7 into the wild-type Bt strain HBF-1 containing the cry8Ca2 gene. The Cry3Aa7 protein produced by strain 3A-HBF was verified by SDS-PAGE and Western blotting. Flat rectangular crystals of Cry3Aa7 protein were observed besides spherical crystals (Cry8Ca2). The plasmid pSTK-3A was stable when strain 3A-HBF was grown in medium without antibiotics. The growth rate of 3A-HBF was not significantly different from that of the recipient strain, HBF-1. Strain 3A-HBF showed toxicity against two families of pests, Scarabaeidae and Chrysomelidae pests, which are susceptible to Cry8Ca (Anomala corpulenta) and Cry3Aa (Leptinotarsa decemlineata and Colaphellus bowringi). The 50% lethal concentrations of 3A-HBF against A. corpulenta, L. decemlineata and C. bowringi were 0.730 x 10(8) c.f.u./g dry soil, 1.74 microg/ml and 1.15 microg/ml, respectively.

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