文献类型： 外文期刊

作者： Liu, Dequan ¹ ; Park, Cheolwoo ¹ ; Wang, Qingyu ² ; Xu, Donghe ¹ ;

作者机构： 1.Japan Int Res Ctr Agr Sci JIRCAS, 1-1 Ohwashi, Tsukuba, Ibaraki 3058686, Japan

2.Jilin Univ, Coll Plant Sci, 5333 Xian Rd, Changchun, Jilin, Peoples R China

3.Jilin Acad Agr Sci, 1363 Shengtai Rd, Changchun, Jilin, Peoples R China

关键词： candidate gene; cultivated soybean; gene expression; near-isogenic lines (NILs); qSW17.1; quantitative trait locus (QTL); seed weight; wild soybean

期刊名称：CROP & PASTURE SCIENCE （ 影响因子：2.249； 五年影响因子：2.541 ）

ISSN： 1836-0947

年卷期：

页码：

收录情况： SCI

摘要： Context: Seed weight is an important agronomic trait for determining yield and appearance quality of soybean (Glycine max (L.) Merr.). Understanding the genetic basis of seed weight might lead to improvement of these traits in soybean by optimising different genes or alleles controlling seed weight. Aims: A major quantitative trait locus (QTL) for seed weight, qSW17.1, was identified previously. In this study, we used progenies of cultivated soybean and wild soybean (Glycine soja Sieb. and Zucc.) for further validation and characterisation of qSW17.1. Methods: A BC4F2 population, a heterogeneous inbred family (HIF) population, and a pair of qSW17.1 near-isogenic lines (NILs) developed from progenies of a cross between cultivated soybean variety Jackson and wild soybean accession JWS156-1 were cultivated under field conditions. QTL analysis and candidate gene mining were conducted. Key results: A QTL corresponding to qSW17.1, which explained 19.84% and 31.71% of the total phenotypic variance in BC4F2 and HIF populations, respectively, was detected. The NIL with the cultivated soybean allele showed higher shoot biomass than the NIL with the wild soybean allele under hydroponic growth conditions, suggesting that the large-seed-size allele of qSW17.1 might be beneficial in soybean seedling establishment. qSW17.1 was delimited to a physical interval of 2515 kb on chromosome 17. Glyma.17G108500 showed a large (similar to 3.27-fold) difference in expression between the two NILs, and was considered a candidate gene underlying qSW17.1.