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Genetic Analysis of S5 Regulating the Hybrid Sterility between Indica and Japonica Subspecies in Rice

文献类型： 外文期刊

作者： Guo, Jie 1 ; Zhou, Xinqiao 1 ; Chen, Ke 1 ; Ye, Chanjuan 1 ; Liu, Juan 1 ; Sun, Kangli 4 ; Tang, Guang 4 ; Wang, Shaokui 4 ; Zhang, Guiquan 4 ; Chen, Youding 1 ; Chen, Dagang 1 ; Liu, Chuanguang 1 ;

作者机构： 1.Minist Agr & Rural Affairs, Coconstruct Minist & Prov, Key Lab Genet & Breeding High Qual Rice Southern C, Guangzhou 510640, Peoples R China

2.Guangdong Key Lab New Technol Rice Breeding, Guangzhou 510640, Peoples R China

3.Guangdong Acad Agr Sci, Rice Res Inst, Guangdong Rice Engn Lab, Guangzhou 510640, Peoples R China

4.South China Agr Univ, Guangdong Prov Key Lab Plant Mol Breeding, Guangzhou 510640, Peoples R China

关键词： hybrid sterility; genetic effect; transcriptome analysis; CRISPR/Cas9; heterosis

期刊名称：AGRONOMY-BASEL （ 影响因子：3.7； 五年影响因子：4.0 ）

ISSN：

年卷期： 2023 年 13 卷 4 期

页码：

收录情况： SCI

摘要： Hybrid sterility is the major obstacle to the utilization of inter-subspecific heterosis in hybrid rice breeding. The S5 locus, composed of three adjacent genes ORF3, ORF4, and ORF5, plays a crucial role in regulating indica/japonica hybrids' female sterility. Through a series of crosses involving 38 parents, three alleles of S5, ORF3+ORF4-ORF5n, ORF3+ORF4+ORF5n, and ORF3-/ORF4-/ORF5n, all could be regarded as wide-compatibility alleles, and when crossed with indica or japonica rice, they all showed significantly high fertility. Then, in order to explore the genes' function, we further knocked out genes by using CRISPR/Cas9-based genome editing. Our results demonstrate that the ORF3+ was not just the protector in the killer-protector system, and knocking out ORF3 of the indica allele seriously affected the rice's normal development. We observed the concrete enhancing hybrid spikelet fertility from the crosses between the ORF4+ knockout japonica materials with indica varieties. By conducting the comparative RNA-Seq analysis of young spikelets, we found that the ORF4+/ORF4- could modulate the hybrid fertility by affecting the expressions of genes related to the function of the Golgi apparatus. This study indicated that knocking out the ORF4+ of the japonica allele or using the alleles carrying ORF5n would provide effective approaches to overcome indica/japonica hybrid female sterility in rice breeding.

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