Artificial Gynogenesis and Sex Determination in Half-Smooth Tongue Sole (Cynoglossus semilaevis)
文献类型： 外文期刊
作者： Chen, Song-Lin 1 ; Tian, Yong-Sheng 1 ; Yang, Jing-Feng 1 ; Shao, Chang-Wei 1 ; Ji, Xiang-Shan 1 ; Zhai, Jie-Ming;
作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China
2.Chinese Acad Fishery Sci, Yellow S
期刊名称：MARINE BIOTECHNOLOGY （ 影响因子：3.619； 五年影响因子：3.322 ）
ISSN：
年卷期：
页码：
收录情况： SCI
摘要： Half-smooth tongue sole (Cynoglossus semilaevis) is an important cultured marine fish as well as a promising model fish for the study of sex determination mechanisms. In the present study, a protocol for artificial gynogenesis of half-smooth tongue sole was developed in order to identify the sex determination mechanism and to generate all-female stock. The optimal UV-irradiation dose for genetically inactivating sea perch spermatozoa was determined to be >/=30 mJ/cm(2). The optimal initiation time for cold shock of gynogenetic embryos was determined to be 5 min after fertilization, while the optimal temperature and treatment duration were determined to be 20-25 min at 5 degrees C. Chromosomes from common diploids, gynogenetic haploids, and diploids were analyzed. WW chromosomes were discovered in some of the gynogenetic diploids. The microsatellite marker was applied to analyze gynogenetic diploid fry. Among the 30 gynogenetic diploid fry, 11 fry contained only one allele, while 19 contained two alleles, which had the same genotype as their mother. The female-specific DNA marker was observed in four individuals out of ten gynogenetic diploid fry. Ploidy analysis of 20 putative gynogenetic fry showed them all to be diploid. Thus, a protocol for the induction of artificial gynogenesis has been developed for the first time in half smooth tongue sole, and the sex determination mechanism in the tongue sole was determined to be female heterogametic with the ZW chromosome.
- 相关文献
作者其他论文 更多>>
-
Decoding the fish genome opens a new era in important trait research and molecular breeding in China
作者：Zhou, Qian;Wang, Jialin;Chen, Zhangfan;Wang, Na;Li, Ming;Wang, Lei;Si, Yufeng;Lu, Sheng;Cui, Zhongkai;Liu, Xuhui;Chen, Songlin;Zhou, Qian;Wang, Jialin;Chen, Zhangfan;Wang, Na;Li, Ming;Wang, Lei;Si, Yufeng;Lu, Sheng;Cui, Zhongkai;Liu, Xuhui;Chen, Songlin;Li, Jiongtang
关键词：fish genomics; economic traits; genomic selection; genome editing; genomic breeding
-
Molecular phylogenetic relationships based on mitochondrial genomes of novel deep-sea corals (Octocorallia: Alcyonacea): Insights into slow evolution and adaptation to extreme deep-sea environments
作者：Wei, Zhan-Fei;Liu, Kai-Qiang;Shao, Chang-Wei;Zhang, Nan-Nan;Liu, Shan-Shan;Meng, Liang;Cai, Chong-Yang;Ta, Kai-Wen;Peng, Xiao-Tong
关键词：Mitochondrial genome; Alcyonacea; Ka/Ks evolution; Environmental factors
-
Three Copies of zbed1 Specific in Chromosome W Are Essential for Female-Biased Sexual Size Dimorphism in Cynoglossus semilaevis
作者：Sun, Yuqi;Sun, Yuqi;Li, Xihong;Mai, Jiaqi;Xu, Wenteng;Wang, Jiacheng;Zhang, Qi;Wang, Na;Mai, Jiaqi;Wang, Jiacheng;Zhang, Qi
关键词：Cynoglossus semilaevis; sexual size dimorphism; zbed1
-
Characterization of emopamil binding protein (Cs-ebp) reveals its potential role in cholesterol biosynthesis and growth performance of Chinese tongue sole
作者：Chen, Zhangfan;Li, Xihong;Wang, Na;Chen, Songlin;Chen, Zhangfan;Li, Xihong;Cheng, Peng;Mai, Jiaqi;Sun, Yuqi;Wang, Jiacheng;Shi, Rui;Zhang, Qi;Wang, Na;Chen, Songlin;Chen, Zhangfan;Li, Xihong;Cheng, Peng;Mai, Jiaqi;Sun, Yuqi;Wang, Jiacheng;Shi, Rui;Zhang, Qi;Wang, Na;Chen, Songlin;Wang, Na;Chen, Songlin
关键词：Chinese tongue sole; Emopamil-binding protein (EBP); Cholesterol biosynthesis; Sexual size dimorphism; Ovarian development
-
Identification and Functional Analysis of Cynoglossus semilaevis Z-Linked E3 Ubiquitin Ligase rnf34
作者：Li, Lu;Li, Lu;Li, Xihong;Chen, Yadong;Yang, Yingming;Wang, Na;Xu, Wenteng
关键词：Cynoglossus semilaevis; ubiquitin ligase; rnf34; spermatogenesis; siRNA-mediated knockdown
-
A comparative study on the measurement of sustainable development of marine fisheries in China
作者：Zhang, Yi-Zhuo;Xue, Cheng;Wang, Na;Chen, Gang
关键词：Marine fishery; Sustainable development; China
-
Establishment, characterization and application of Largemouth bronze gudgeon ( Coreius guichenoti ) fin and skin cell lines in disease resistance
作者：Zhang, Qi;Shi, Yuhong;Liao, Xiaolin;Tian, Hua;Zhang, Qi;Gong, Zhihong;Shi, Rui;Shi, Yuhong;Guo, Chenfei;Wang, Na;Gong, Zhihong
关键词：Largemouth bronze gudgeon(Coreius guichenoti); Cell lines; Skin; Fin; Spring viremia of carp virus (SVCV)
