Delta(6) Fatty Acid Desaturase Gene in Estuarine Tapertail Anchovy (Coilia nasus): Structure Characterization and mRNA Expression under Different Dietary and Fasting Conditions
文献类型： 外文期刊
作者： Duan, Jinrong 1 ; Wei, Guanglian 1 ; Xu, Dongpo 1 ; Liu, Kai 1 ; Fang, Dian 1 ; Xu, Pao 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Sci Observing & Expt Stn Fishery Resources & Envi, Freshwater Fisheries Res Ctr, Wuxi 214081, Peoples R China
关键词： Coilia nasus; Delta 6 fatty acid desaturases; gene characterization; mRNA expression; lipid metabolism enzyme; artificial diets
期刊名称：PAKISTAN JOURNAL OF ZOOLOGY （ 影响因子：0.831； 五年影响因子：0.768 ）
ISSN： 0030-9923
年卷期： 2016 年 48 卷 4 期
页码：
收录情况： SCI
摘要： The enzyme Delta(6)-fatty acid desaturase regulates the rate of highly unsaturated fatty acid (HUFA) biosynthesis. We first obtained the cDNA sequences and structures of Fads6 in Coilia nasus and investigated the mRNA expression patterns in tissue, and in juveniles under different dietary regulation. The Fads6 cDNA was 2032 bp, specifying a protein of 445 amino acids. A high expression was observed in the brain and intestine, relatively high expression in muscle and liver, and a weak expression was exhibited in the heart, kidney and gill. The effects of three different diets (diet 1-soft pellet; diet 2-slow-sinking pellet; diet 3- Daphnia) were studied on lipid metabolism enzyme activities and gene expression levels in different tissues of juveniles of C. nasus for 60 d. The differential expression of Fads6 genes in liver, brain and intestine changed with different diets, respectively. We detected lipid metabolism enzyme-specific activities such as lipase enzyme, lipoprotein lipase enzyme and lipid peroxides in liver, and found that the lipase activity in liver of diet 2 group was significantly (P<0.05) higher than the other diets. The mechanism of HUFAs synthesis in C. nasus is described in the future.
- 相关文献
作者其他论文 更多>>
-
Comparative transcriptome analysis of gill tissue response to hypoxia stress in the Chinese mitten crab ( Eriocheir sinensis)
作者：Chen, Xue;Bai, Yulin;Wang, Anqi;Xu, Dongpo;Ma, Junlei;Xu, Dongpo
关键词：Transcriptome; Eriocheir sinensis; Hypoxia; Oxidative stress; Apoptosis
-
Application of the European Non-native Species in Aquaculture Risk Analysis Scheme to evaluate Micropterus nigricans in China
作者：Li, Shan;Li, Fan;Li, Shan;Li, Fan;Wei, Hui;Gu, Dangen;Wei, Hui;Gu, Dangen;Wei, Hui;Gu, Dangen;Kang, Bin;Xu, Dongpo;Xu, Xiaojun;Stauffer, Jay R.;Stauffer, Jay R.
关键词：aquacultural species; risk assessment; freshwater fish; AS-ISK; ENSARS
-
Temporal-Spatial Variations in Physicochemical Factors and Assessing Water Quality Condition in River-Lake System of Chaohu Lake Basin, China
作者：Wu, Li;Wang, Ziqi;Yang, Yujie;Sang, Rui;Zhu, Haoyue;Wang, Xitong;Pang, Yuqing;Tong, Jiangshan;Liu, Xiangting;Ma, Mingyue;Wang, Qianqian;Ma, Kaijun;Liu, Fan;Liu, Kai;Liu, Kai
关键词：water quality index; trophic level index; Chaohu Lake; spatiotemporal water quality pattern
-
Development and validation of a 20 K genome-wide liquid SNP array for Chinese mitten crab (Eriocheir sinensis) breeding and germplasm identification
作者：Bai, Yulin;Jiang, Zhou;Wang, Anqi;Hu, Changsui;Xu, Dongpo;Bai, Yulin;Ma, Junlei;Wang, Anqi;Hu, Changsui;Xu, Dongpo;Liang, Yidong;Zhang, Ting;Xu, Dongpo
关键词：Eriocheir sinensis; Genome resequencing; Liquid SNP array; GWAS; Phylogenetic analysis
-
Chromosome-level genome assembly of the freshwater bivalve Anodonta woodiana
作者：Chen, Xiubao;Jiang, Tao;Xue, Junren;Liu, Kai;Gu, Mengying;Wang, Meiyi
关键词：
-
Genome-wide identification and characterization of the carbonic anhydrase gene family reveals carbonate alkalinity adaptability in Chinese mitten crab (Eriocheir sinensis)
作者：Bai, Yulin;Wang, Anqi;Xu, Dongpo;Bai, Yulin;Ma, Junlei;Wang, Anqi;Xu, Dongpo;Fan, Zihan
关键词：Carbonic anhydrase; Eriocheir sinensis; Gene structure; Phylogenetic analysis; Subcellular localization
-
Estuarine plastic dynamics: Analyzing export patterns from a typical semi-enclosed bay in Asia
作者：Wei, Nian;Lu, Jungang;Jiao, Meng;Zong, Changxing;Zhang, Feng;Jiang, Chunhua;Zhu, Lixin;Liu, Kai;Cui, Tiefeng;Li, Daoji;Wei, Nian;Lu, Jungang;Jiao, Meng;Zong, Changxing;Zhang, Feng;Jiang, Chunhua;Zhu, Lixin;Liu, Kai;Cui, Tiefeng;Li, Daoji;Wei, Nian;Lu, Jungang;Jiao, Meng;Zong, Changxing;Zhang, Feng;Jiang, Chunhua;Zhu, Lixin;Liu, Kai;Cui, Tiefeng;Li, Daoji;Wei, Nian;Liu, Kai
关键词：Microplastic (MPs); Spatial distribution; Principal Coordinate Analysis (PCoA); Semi-enclosed bays; Hydrodynamic processes; Pollution management
