Identification and characterization of a progestin and adipoQ receptor (PAQR) structurally related to Paqr7 in the ovary of Cynoglossus semilaevis and its potential role in regulating oocyte maturation
文献类型： 外文期刊
作者： Shi, Bao 1 ; Liu, Xuezhou 1 ; Thomas, Peter 3 ; Pang, Yefei 3 ; Xu, Yongjiang 1 ; Li, Xiaoni 1 ; Li, Xiaoxiao 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Dev Marine Fisheries, Minist Agr, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266071, Peoples R China
3.Univ Texas Austin, Inst Marine Sci, Port Aransas, TX 78373 USA
关键词： Cynoglossus semilaevis; Progestin and adipoQ receptor-related to mPR alpha (Paqr7b); Expression; Reproduction; Oocyte maturation
期刊名称：GENERAL AND COMPARATIVE ENDOCRINOLOGY （ 影响因子：2.822； 五年影响因子：2.772 ）
ISSN： 0016-6480
年卷期： 2016 年 237 卷
页码：
收录情况： SCI
摘要： Membrane progestin receptors (mPRs) play an important role in the regulation of oocyte meiotic maturation in fish. However, details of the molecular endocrine mechanism regulating oocyte maturation in multiple spawning fish with asynchronous ovarian development remain unclear. The cDNA encoding a novel progestin and adipoQ receptor with structural similarity to mPR alpha (Paqr7), herein called Paqr7b, was cloned and sequenced from the ovary of half-smooth tongue sole Cynoglossus semilaevis. Phylogenetic analysis showed that Paqr7b represents an evolutionary intermediate between mPR alpha and mPR beta and shares high homology with other similar Paqr proteins in other teleost species. However, the tongue sole Paqr7b protein showed much greater homology to teleost mPR alpha s (average 52%) than mPR beta s (average 40%), suggesting it may have arisen from gene duplication of mPR alpha. paqr7b and paqr7 mRNA exhibited similar patterns of tissue expression. The mRNA and protein of Paqr7b were ubiquitously detected in all tissues analyzed, including the ovary. Moreover, in situ hybridization results revealed that paqr7b was expressed in stage V oocytes, as well as in scattered cells in the pituitary. The expression of paqr7b mRNA in brain and ovary significantly increased from ovarian development stage II to stage V (P < 0.05), and was maximal at stage V, and then sharply decreased at stage VI. The transcript level of paqr7b mRNA in the pituitary also peaked at stage V (P < 0.05). Treatment of tongue sole ovarian follicles with gonadotropin consistently increased the expression level of Paqr7b protein and mRNA in both a dose- and stage-dependent manner. Microinjection of tongue sole oocytes with a morpholino antisense oligonucleotide to Paqr7b blocked the progestin induction of oocyte maturation. Our findings demonstrate an important role of Paqr7b in the regulation of oocyte maturation in tongue sole and suggest the receptor may also influence other aspects of reproduction, such as pituitary function. (C) 2016 Elsevier Inc. All rights reserved.
- 相关文献
作者其他论文 更多>>
-
High-quality chromosome-level genome assembly of the whitespotted conger (Conger myriaster)
作者：Han, Shenglei;Han, Shenglei;Li, Chen;Li, Shuo;Chen, Yan;Liu, Kaiqiang;Liu, Yuyan;Wang, Hong-Yan;Wang, Qian;Cao, Xuwen;Shi, Bao;Shao, Changwei;Han, Shenglei;Li, Chen;Li, Shuo;Chen, Yan;Liu, Kaiqiang;Liu, Yuyan;Wang, Hong-Yan;Wang, Qian;Cao, Xuwen;Shi, Bao;Shao, Changwei;Wang, Chenggang;Liu, Kaiqiang;Liu, Yuyan;Wang, Hong-Yan;Wang, Qian;Cao, Xuwen;Shao, Changwei
关键词：
-
Identification of functional ncRNAs in the liver reveals a novel regulatory mechanism associated with the dynamic balance of growth and reproduction in Cynoglossus semilaevis
作者：Yan, Kewen;Shi, Bao;Chen, Yan;Zhang, Daiqiang;Dong, Yani;Li, Jing;Zhao, Xinyu;Tao, Meijun;Yan, Kewen;Cheng, Hanliang
关键词：Integrated analysis; Growth; Reproduction; Dynamic balance; Cynoglossus semilaevis
-
Effects of leptin on fatty acid metabolism in the liver and reproductive function of tongue sole (Cynoglossus semilaevis) in vivo
作者：Cai, Xin;Li, Yuting;Meng, Zhaojun;Cui, Aijun;Jiang, Yan;Xu, Yongjiang;Meng, Zhaojun
关键词：Leptin; Tongue sole; Lipid metabolism; Regulatory mechanism; Reproductive function
-
The effects of 5-Hydroxymethylfurfural in mitigating transportation stress of large yellow croaker (Larimichthys crocea)
作者：Zheng, Jichang;Feng, Yuan;Xu, Yongjiang
关键词：Larimichthys crocea; 5-Hydroxymethylfurfural; Transportation stress; PI3K-AKT signaling pathway; Heat shock protein 90 alpha
-
Heavy metal distribution and ecological risk in surface sediments of the Bohai Sea
作者：Li, Shilin;Zhang, Jianshe;Li, Shilin;Chen, Jianlei;Zhang, Xuzhi;Xu, Yongjiang;Xu, Yong;Li, Shilin;Chen, Jianlei;Zhang, Xuzhi;Xu, Yongjiang;Xu, Yong
关键词：
-
Yellowtail kingfish Seriola aureovittata farmed in offshore net cage response to high density by regulating protein and glucose metabolism
作者：Jiang, Yan;Xu, Yongjiang;Cui, Aijun;Wang, Kaijie;Wang, Bin;Yu, Chaoyong;Xue, Zhiyong
关键词：
Seriola aureovittata ; Stocking density; Crowding stress; Growth performance; Physiological parameter -
Biogeographic Variation Analysis of Phenotypic and Nutritional Quality Traits of Cultured Conger myriaster Along the Yellow Sea Coast of China
作者：Chen, Yan;Tao, Meijun;Shi, Bao;Han, Shenglei;Liu, Binghua;Yan, Kewen;Zhao, Xinyu;Liu, Mingze;Chen, Yan;Tao, Meijun;Shi, Bao;Han, Shenglei;Liu, Binghua;Yan, Kewen;Zhao, Xinyu;Liu, Mingze;Chen, Yan;Wang, Lianshun;Tao, Meijun
关键词：Conger myriaster; captive population; quantitative and qualitative traits; various regions
