文献类型： 外文期刊
作者： Ke, Xiaoli 1 ; Lu, Maixin 1 ; Wang, Jianguo 2 ;
作者机构： 1.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Key Lab Trop & Subtrop Fishery Resource Applicat, Minist Agr, Guangzhou, Guangdong, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Lab Hlth Aquaculture, Wuhan, Peoples R China
关键词： Fish disease; fungi; molecular diagnosis; zebrafish
期刊名称：JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION （ 影响因子：1.279； 五年影响因子：1.522 ）
ISSN： 1040-6387
年卷期： 2016 年 28 卷 6 期
页码：
收录情况： SCI
摘要： Although Fusarium sp. infections have been reported in aquatic invertebrates, studies of Fusarium spp. as fish pathogens remain very limited. In our study, a fungus was isolated from diseased zebrafish (Danio rerio). DNA sequence analysis of the fungus, based on a partial region of the translation elongation factor 1 gene (EF-1), the internal transcribed spacer region and domains D1 and D2 of the large subunit of the ribosomal RNA gene (ITS plus LSU), and the RNA polymerase II subunit gene (RPB2), showed 99.9-100% homology to Fusarium solani species complex sequences. Multilocus sequence typing analysis based on 3-locus haplotypes (EF-1, ITS plus LSU, and RPB2) suggests that the isolated strain was type 3+4-P. Challenge experiments showed that this organism could be pathogenic to zebrafish, but usually does not infect healthy subjects under normal circumstances.
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