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Effects of methomyl on steroidogenic gene transcription of the hypothalamic-pituitary-gonad-liver axis in male tilapia

文献类型： 外文期刊

作者： Meng, ShunLong 1 ; Qiu, LiPing 1 ; Hu, GengDong 1 ; Fan, LiMin 1 ; Song, Chao 1 ; Zheng, Yao 1 ; Wu, Wei 1 ; Qu, JianHong 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Lab Qual & Safety Risk Assessment Aquat Prod Envi, Minist Agr, 9 East Shanshui Rd, Wuxi 214081, Jiangsu, Peoples R China

2.CAFS, Key Lab Fishery Ecoenvironm Assessment & Resource, Wuxi 214081, Peoples R China

关键词： Methomyl; Hypothalamic-pituitary-gonad-liver axis; Male tilapia; Steroidogenic gene transcription; Recovery pattern

期刊名称：CHEMOSPHERE （ 影响因子：7.086； 五年影响因子：6.956 ）

ISSN： 0045-6535

年卷期： 2016 年 165 卷

页码：

收录情况： SCI

摘要： Male tilapia were exposed to sub-lethal methomyl concentrations of 0, 0.2, 2, 20 or 200 mu g/L. for 30 d, and were subsequently cultured in methomyl-free water for 18 d. Relative transcript abundance of steroidogenic genes involved in the HPGL axis of male tilapia was examined at 30 d in the exposure test and at 18 d in the recovery test. The results revealed that low concentrations of methomyl (0.2 and 2 mu g/L) did not cause significant changes in gene mRNA levels in the HPGL axis of male tilapia; thus, we considered 2 mu g/L concentrations as the level that showed no apparent adverse endocrine disruption effects. However, higher concentrations of methomyl (20 and 200 mu g/L) disrupted the endocrine system and caused significant increase in the levels of GnRH2, GnRH3, ER alpha, and ER beta 3 genes in the hypothalamus, GnRHR and FSH beta genes in the pituitary, CYP19a, FSHR, and ER alpha genes in the testis, and VTG and ER alpha genes in the liver, and significantly decreased the levels of LHR, StAR, 3 beta-HSD, and AR alpha genes in the testis and LH beta gene in the pituitary, leading to changes in sex steroid hormone and vitellogenin levels in the serum and ultimately resulting in reproductive dysfunction in male tilapia. The recovery tests showed that the toxicity effect caused by 20 mu g/L methomyl was reversible; however, the toxicity effect at 200 mu g/L of methomyl was irreversible after 18 d. Therefore, we concluded that 200 mu g/L was the threshold concentration for methomyl-induced irreversible endocrine disruption in male tilapia. (C) 2016 Elsevier Ltd. All rights reserved.

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