Development of a vaccine against Streptococcus agalactiae in fish based on truncated cell wall surface anchor proteins
文献类型： 外文期刊
作者： Liu, H. 1 ; Zhang, S. 3 ; Shen, Z. 3 ; Ren, G. 2 ; Liu, L. 3 ; Ma, Y. 3 ; Zhang, Y. 1 ; Wang, W. 4 ;
作者机构： 1.Southwest Univ, Sch Life Sci, Key Lab Freshwater Fish Reprod & Dev, Key Lab Aquat Sci Chongqing, Chongqing 400715, Peoples R China
2.Binzhou Marine Environm Monitoring Stn, Binzhou 256600, Shandong, Peoples R China
3.Shandong Lvdu Biotechnol Co Ltd, Shandong Binzhou Anim Sci & Vet Med Acad, Binzhou 256600, Shandong, Peoples R China
4.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China
期刊名称：VETERINARY RECORD （ 影响因子：2.695； 五年影响因子：2.797 ）
ISSN： 0042-4900
年卷期： 2016 年 179 卷 14 期
页码：
收录情况： SCI
摘要： Streptococcus agalactiae is an important fish pathogen and a leading cause of major economic losses to the aquaculture industry worldwide. In the present study, the two truncated recombinant proteins of cell wall surface anchor family of S agalactiae, CWSAP465 and CWSAP1035, were expressed in Escherichia coli, and their immunogenicity and efficacy against the bacterium were evaluated in tilapia and turbot. The results showed that the prokaryotic expression of the two constructs, p32a-CWSAP465 and p32a-CWSAP1035, gave rise to a high yield of soluble proteins with good immunogenicity. The immunisation-challenge study revealed that tilapia and turbot immunised with recombinant truncated proteins produced high levels of antibodies with a peak at four weeks after immunisation and were protected from a challenge by a virulent S agalactiae at a dose of 1x10(9) colony forming units/ml. The recombinant truncated proteins had higher efficacy than the whole-cell inactivated vaccine. Therefore, the study demonstrated that CWSAP465 and CWSAP1035 are two viable vaccine candidates against S agalactiae in fish.
- 相关文献
作者其他论文 更多>>
-
EFFECT OF PLANTING LOTUSES ON THE COMMUNITY STRUCTURE OF PLANKTONIC EUKARYOTES IN A SHALLOW POND OF A SUBTROPICAL URBAN PARK IN CHINA
作者：Li, X. W.;Wang, W.;Liu, W. H.;Wu, Q. S.;Peng, W. H.;Zhou, Y. L.;Mo, J. C.;Li, X. W.;Wang, W.;Liu, W. H.;Wu, Q. S.;Peng, W. H.;Zhou, Y. L.;Mo, J. C.;Liu, Q. F.
关键词：artificial ecological modification; freshwater; aquatic microbial ecology; microbial; interactions; Shannon index
-
Mitogenome Characterization of a Vulnerable Gastromyzontid Fish, Beaufortia pingi (Teleostei: Gastromyzontidae): Genome Description and Phylogenetic Considerations
作者：Shen, Z.;Sheng, Q.;Zhang, Y.;Lv, H.;Jin, Z.
关键词：Beaufortia pingi; hillstream loach; mitochondrial genome; phylogenetic analysis
-
Shape variation in the carapace of Chinese mitten crabs (Eriocheir sinensis H. Milne Edwards, 1853) in Yangcheng Lake during the year-long culture period
作者：Xue, J.;Yang, J.;Liu, H.;Jiang, T.;Chen, X.;Yang, J.
关键词：Yangcheng Lake; eriocheir sinensis; geometric morphometric analysis; landmark; ontogeny
-
ToxR modulates biofilm formation in fish pathogen Vibrio harveyi
作者：Zhang, Y.;Deng, Y.;Feng, J.;Hu, J.;Chen, H.;Guo, Z.;Su, Y.;Zhang, Y.;Hu, J.;Chen, H.;Gao, R.;Su, Y.
关键词：biofilm formation; gene knockout; phenotypic characterization; toxR; Vibrio harveyi
-
Joint multiple quantitative trait loci mapping for allometries of body compositions and metabolic traits to body weights in broiler
作者：Zhou, X.;Zhang, H.;Ye, J.;Xu, Y.;Zhou, X.;Ye, J.;Xu, Y.;Zhang, Y.;Du, J.;Yang, R.
关键词：relative growth; random regression model; organ tissue; quality trait; linkage analysis
-
Effect of replacing fish meal with meat and bone meal on growth, feed utilization and nitrogen and phosphorus excretion for juvenile Pseudobagrus ussuriensis
作者：Tang, B.;Bu, X.;Lian, X.;Zhang, Y.;Yang, Y.;Muhammad, I.;Zhou, Q.;Liu, H.
关键词：apparent digestibility coefficients; fish meal; growth; meat and bone meal; nitrogen and phosphorus excretion; Pseudobagrus ussuriensis
-
Length-weight relationships of six fishes from the Anning River, China
作者：Ru, H. J.;Zhang, Y.;Li, Y. F.;Wu, X. X.;Ni, Z. H.
关键词：
